TY - JOUR
TI - Amino acid and fatty acid levels affect hepatic phosphorus metabolite content in metabolically healthy humans
AU - Kahl, S.
AU - Nowotny, B.
AU - Strassburger, K.
AU - Bierwagen, A.
AU - Klüppelholz, B.
AU - Hoffmann, B.
AU - Giani, G.
AU - Nowotny, P.J.
AU - Wallscheid, F.
AU - Hatziagelaki, E.
AU - Pacini, G.
AU - Hwang, J.-H.
AU - Roden, M.
JO - JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM
PY - 2018
VL - 103
TODO - 2
SP - 460-468
PB - Oxford University Press
SN - 0021-972X
TODO - 10.1210/jc.2017-01773
TODO - adenosine triphosphate;  alanine;  amino acid;  arginine;  citrulline;  fatty acid;  glycine;  histidine;  isoleucine;  leucine;  lysine;  methionine;  ornithine;  palmitoleic acid;  phenylalanine;  phosphate;  phosphorus;  proline;  serine;  threonine;  tryptophan;  tyrosine;  valine;  amino acid;  fatty acid;  phosphorus, adult;  age;  aged;  Article;  body mass;  female;  glucose blood level;  human;  human experiment;  insulin sensitivity;  male;  metabolome;  normal human;  nuclear magnetic resonance imaging;  oral glucose tolerance test;  phosphorus nuclear magnetic resonance;  priority journal;  proton nuclear magnetic resonance;  sex;  cohort analysis;  energy metabolism;  feasibility study;  glucose tolerance test;  health;  liver;  metabolism;  middle aged;  physiology;  young adult, Adult;  Aged;  Amino Acids;  Cohort Studies;  Energy Metabolism;  Fatty Acids;  Feasibility Studies;  Female;  Glucose Tolerance Test;  Health;  Humans;  Liver;  Male;  Metabolome;  Middle Aged;  Phosphorus;  Young Adult
TODO - Objective: Hepatic energy metabolism negatively relates to insulin resistance and liver fat content in patients with type 2 diabetes, but its role inmetabolically healthy humans is unclear.Wehypothesized that intrahepatocellular g-Adenosine triphosphate (gATP) and inorganic phosphate (Pi) concentrations exhibit similar associations with insulin sensitivity in nondiabetic, nonobese volunteers. Design:Atotal of 76 participantsunderwent a four-point sampling, 75-goralglucose tolerance test (OGTT), as well as in vivo 31P/1Hmagnetic resonance spectroscopy. In 62 of them, targeted plasmametabolomic profiling was performed. Pearson correlation analyses were performed for the dependent variables gATP and Pi. Results: Adjusted for age, sex, and body mass index (BMI), hepatic gATP and Pi related to 2-hour OGTT glucose (r = 0.25 and r = 0.27, both P<0.05), and Pi further associated with nonesterified fatty acids (NEFAs; r = 0.28, P < 0.05). However, neither gATP nor Pi correlated with several measures of insulin sensitivity. Hepatic gATP correlated with circulating leucine (r = 0.42, P< 0.001) and Pi with C16:1 fatty acids palmitoleic acid and C16:1w5 (r = 0.28 and 0.30, respectively, P< 0.01), as well as with d-9-desaturase index (r = 0.33, P <0.05). Only the association of gATP with leucine remained important after correction for multiple testing. Leucine and palmitoleic acid, together with age, sex, and BMI, accounted for 26% and for 15% of the variabilities in gATP and Pi, respectively. Conclusions: Specific circulating amino acids and NEFAs, but notmeasures of insulin sensitivity, partly affect hepatic phosphorus metabolites, suggesting mutual interaction between hepatic energy metabolism and circulating metabolites in nondiabetic humans. Copyright © 2018 Endocrine Society.
ER -