TY - JOUR
TI - The family of 14-3-3 proteins and specifically 14-3-3σ are up-regulated during the development of renal pathologies
AU - Rizou, M.
AU - Frangou, E.A.
AU - Marineli, F.
AU - Prakoura, N.
AU - Zoidakis, J.
AU - Gakiopoulou, H.
AU - Liapis, G.
AU - Kavvadas, P.
AU - Chatziantoniou, C.
AU - Makridakis, M.
AU - Vlahou, A.
AU - Boletis, J.
AU - Vlahakos, D.
AU - Goumenos, D.
AU - Daphnis, E.
AU - Iatrou, C.
AU - Charonis, A.S.
JO - Journal of Cellular and Molecular Medicine
PY - 2018
VL - 22
TODO - 9
SP - 4139-4149
PB - Blackwell Publishing Inc.
SN - 1582-1838, 1582-4934
TODO - 10.1111/jcmm.13691
TODO - calreticulin;  complementary DNA;  immunoglobulin A;  messenger RNA;  peptides and proteins;  protein 14 3 3;  stratifin;  transcription factor HIF1 alpha;  unclassified drug;  calreticulin;  calreticulin, human;  exoribonuclease;  HIF1A protein, human;  hypoxia inducible factor 1alpha;  isoenzyme;  protein 14 3 3;  SFN protein, human;  tumor marker, animal experiment;  animal model;  animal tissue;  Article;  cell culture;  chromatin immunoprecipitation;  controlled study;  densitometry;  experimental glomerulonephritis;  gene overexpression;  homeostasis;  housekeeping gene;  human;  human cell;  hypoxia;  immunoglobulin A nephropathy;  immunohistochemistry;  kidney fibrosis;  kidney tubule epithelium;  male;  matrix assisted laser desorption ionization time of flight mass spectrometry;  mouse;  nephrectomy;  nephrotoxicity;  nonhuman;  priority journal;  protein expression;  proteomics;  real time polymerase chain reaction;  reperfusion injury;  upregulation;  ureter obstruction;  Western blotting;  animal;  C57BL mouse;  cell line;  chronic kidney failure;  disease model;  epithelium cell;  fibrosis;  gene expression regulation;  genetics;  kidney tubule;  membranous glomerulonephritis;  metabolism;  pathology;  procedures;  promoter region;  signal transduction, 14-3-3 Proteins;  Animals;  Biomarkers, Tumor;  Calreticulin;  Cell Line;  Disease Models, Animal;  Epithelial Cells;  Exoribonucleases;  Fibrosis;  Gene Expression Regulation;  Glomerulonephritis, IGA;  Glomerulonephritis, Membranous;  Humans;  Hypoxia-Inducible Factor 1, alpha Subunit;  Isoenzymes;  Kidney Tubules;  Male;  Mice;  Mice, Inbred C57BL;  Promoter Regions, Genetic;  Proteomics;  Renal Insufficiency, Chronic;  Reperfusion Injury;  Signal Transduction;  Ureteral Obstruction
TODO - Chronic kidney disease, the end result of most renal and some systemic diseases, is a common condition where renal function is compromised due to fibrosis. During renal fibrosis, calreticulin, a multifunctional chaperone of the endoplasmic reticulum (ER) is up-regulated in tubular epithelial cells (TECs) both in vitro and in vivo. Proteomic analysis of cultured TECs overexpressing calreticulin led to the identification of the family of 14-3-3 proteins as key proteins overexpressed as well. Furthermore, an increased expression in the majority of 14-3-3 family members was observed in 3 different animal models of renal pathologies: the unilateral ureteric obstruction, the nephrotoxic serum administration and the ischaemia-reperfusion. In all these models, the 14-3-3σ isoform (also known as stratifin) was predominantly overexpressed. As in all these models ischaemia is a common denominator, we showed that the ischaemia-induced transcription factor HIF1α is specifically associated with the promoter region of the 14-3-3σ gene. Finally, we evaluated the expression of the family of 14-3-3 proteins and specifically 14-3-3σ in biopsies from IgA nephropathy and membranous nephropathy patients. These results propose an involvement of 14-3-3σ in renal pathology and provide evidence for the first time that hypoxia may be responsible for its altered expression. © 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
ER -