TY - JOUR
TI - Polymorphisms of cystathionine beta-synthase gene are associated with susceptibility to sepsis
AU - Sponholz, C.
AU - Kramer, M.
AU - Schöneweck, F.
AU - Menzel, U.
AU - Rahatloo, K.I.
AU - Giamarellos-Bourboulis, E.J.
AU - Papavassileiou, V.
AU - Lymberopoulou, K.
AU - Pavlaki, M.
AU - Koutelidakis, I.
AU - Perdios, I.
AU - Scherag, A.
AU - Bauer, M.
AU - Platzer, M.
AU - Huse, K.
JO - European Journal of Human Genetics: EJHG
PY - 2016
VL - 24
TODO - 7
SP - 1041-1048
PB - Nature Publishing Group
SN - 1018-4813, 1476-5438
TODO - 10.1038/ejhg.2015.231
TODO - cystathionine beta synthase;  homocysteine;  cystathionine beta synthase, adult;  alternative RNA splicing;  Article;  chimpanzee;  controlled study;  disease severity;  exon;  female;  gene linkage disequilibrium;  gene structure;  genetic association;  genetic risk;  genetic susceptibility;  genetic variability;  genotype;  Germany;  gorilla;  Greece;  haplotype;  heterozygosity;  human;  human cell;  Hylobatidae;  infection sensitivity;  intron;  major clinical study;  male;  nonhuman;  priority journal;  rhesus monkey;  sepsis;  septic shock;  single nucleotide polymorphism;  variable number of tandem repeat;  aged;  case control study;  copy number variation;  genetics;  HEK293 cell line;  middle aged;  pathology;  septic shock;  tumor cell line, Aged;  Alternative Splicing;  Case-Control Studies;  Cell Line, Tumor;  Cystathionine beta-Synthase;  DNA Copy Number Variations;  Female;  HEK293 Cells;  Humans;  Linkage Disequilibrium;  Male;  Middle Aged;  Minisatellite Repeats;  Polymorphism, Single Nucleotide;  Shock, Septic
TODO - Sepsis is the systemic inflammatory host response to infection. Cystathionine beta-synthase (CBS)-dependent homocysteine (Hcy) pathway was demonstrated to affect disease severity and mortality in patients with severe sepsis/septic shock. Independent studies identified a single-nucleotide polymorphism (SNP, rs6586282, hg19 chr21:g.44478497C4T) in intron 14 of the CBScoding gene (CBS) associated with Hcy plasma levels. We aimed to describe the association of this SNP and variants of a splice donor-affecting variable-number tandem repeat (VNTR, NG-008938.1:g.22763-22793[16-22]) 243 bp downstream of rs6586282 with severe human sepsis. We analyzed the VNTR structure and genotyped variants of rs6586282 and a neighboring SNP (rs34758144, hg19 chr21:g.44478582G4A) in two case-control studies including patients with severe sepsis/septic shock from Germany (n=168) and Greece (n=237). In both studies, we consistently observed an association of CBS VNTR alleles with sepsis susceptibility. Risk linearly increased with number of tandem repeats (per allele odds ratio in the adjusted analysis 1.34; 95% confidence interval (CI)=1.17-1.55; Po0.001). Association had also been shown for rs34758144 whose risk allele is in linkage disequilibrium with one long VNTR allele (19 repeat). In contrast, we observed no evidence for an effect on 28-day survival in patients with severe sepsis/septic shock (per allele hazard ratio in the adjusted analysis for VNTR 1.10; 95% CI=0.95-1.28; P=0.20). In a minigene approach, we demonstrated alternative splicing in distinct VNTR alleles, which, however, was independent of the number of tandem units. In conclusion, there is no ordinary conjunction between human CBS and severe sepsis/septic shock, but CBS genotypes are involved in disease susceptibility. © 2016 Macmillan Publishers Limited. All rights reserved.
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