TY - JOUR
TI - Expression analysis of mir-17-5p, mir-20a and let-7a microRNAs and their target proteins in CD34+ bone marrow cells of patients with myelodysplastic syndromes
AU - Vasilatou, D.
AU - Papageorgiou, S.G.
AU - Kontsioti, F.
AU - Kontos, C.K.
AU - Tsiotra, P.
AU - Mpakou, V.
AU - Pavlou, M.A.S.
AU - Economopoulou, C.
AU - Dimitriadis, G.
AU - Dervenoulas, J.
AU - Pappa, V.
JO - Leukemia Research
PY - 2013
VL - 37
TODO - 3
SP - 251-258
PB - 
SN - 0145-2126
TODO - 10.1016/j.leukres.2012.11.011
TODO - cyclin D1;  K ras protein;  microRNA;  microRNA 17 5p;  microRNA 20a;  microRNA let 7a;  Myc protein;  protein bcl 2;  transcription factor E2F1;  unclassified drug, adult;  article;  bone marrow cell;  CD34 selection;  clinical article;  controlled study;  cytogenetics;  down regulation;  female;  high risk patient;  human;  human cell;  karyotype;  low risk population;  male;  myelodysplastic syndrome;  overall survival;  priority journal;  prognosis;  protein expression;  protein targeting;  real time polymerase chain reaction;  Western blotting, Aged;  Aged, 80 and over;  Antigens, CD34;  Bone Marrow Cells;  Case-Control Studies;  Female;  Gene Expression Profiling;  Gene Expression Regulation;  Humans;  Male;  MicroRNAs;  Middle Aged;  Myelodysplastic Syndromes;  Prognosis
TODO - Mir-17-5p and mir-20a, members of the mir-17-92 family, down-regulate E2F1, which is over-expressed in myelodysplastic syndromes (MDS). Moreover, let-7a down-regulates KRAS, which is aberrantly expressed in MDS. We evaluated the expression of the aforementioned microRNAs in CD34+ cells of 43 MDS patients using real-time PCR and their target proteins (E2F1, MYC, BCL2, CCND1, and KRAS) by Western blot. Mir-17-5p and mir-20a were under expressed in high risk MDS patients, compared to low risk MDS patients. Similarly, let-7a was under expressed in patients with intermediate or high-risk karyotype. Interestingly, there was an inverse correlation between microRNA and the expression levels of their targets. Importantly, mir-17-5p and mir-20a constitute favorable prognostic factors in MDS, since their expression was associated with increased overall survival of MDS patients. © 2012 Elsevier Ltd.
ER -