TY - JOUR
TI - Effect of BRCA1 immunohistochemical localizations on prognosis of patients with sporadic breast carcinomas
AU - Mylona, E.
AU - Melissaris, S.
AU - Nomikos, A.
AU - Theohari, I.
AU - Giannopoulou, I.
AU - Tzelepis, K.
AU - Nakopoulou, L.
JO - PATHOLOGY RESEARCH AND PRACTICE
PY - 2014
VL - 210
TODO - 8
SP - 533-540
PB - Urban und Fischer Verlag GmbH und Co. KG
SN - 0344-0338
TODO - 10.1016/j.prp.2014.05.009
TODO - BRCA1 protein;  epidermal growth factor receptor 2;  estrogen receptor;  Ki 67 antigen;  progesterone receptor;  protein p53;  BRCA1 protein;  epidermal growth factor receptor 2;  estrogen receptor;  progesterone receptor, adult;  aged;  article;  breast carcinoma;  cancer cell line;  cancer localization;  cancer prognosis;  cancer tissue;  cell nucleus;  controlled study;  cytoplasm;  disease association;  female;  human;  human cell;  human tissue;  immunohistochemistry;  immunoreactivity;  lymph node metastasis;  major clinical study;  morphometrics;  overall survival;  protein expression;  protein interaction;  recurrence free survival;  breast tumor;  metabolism;  middle aged;  Paget nipple disease;  pathology;  prognosis;  very elderly, Adult;  Aged;  Aged, 80 and over;  BRCA1 Protein;  Breast Neoplasms;  Carcinoma, Ductal, Breast;  Female;  Humans;  Immunohistochemistry;  Middle Aged;  Prognosis;  Receptor, ErbB-2;  Receptors, Estrogen;  Receptors, Progesterone
TODO - Our purpose was to investigate the expression pattern of BRCA1 protein in sporadic breast carcinomas, as well as the clinicopathological and prognostic value of its subcellular localizations. Immunohistochemistry was performed on paraffin embedded tissue specimens from 111 sporadic, invasive breast carcinomas to detect the expression of the proteins BRCA1, ER, PR, erbB2, p53 and Ki67. BRCA1 protein was detected in the nuclei and the cytoplasm of the tumor cells. Nuclear BRCA1 immunoreactivity showed no relation with the classic clinicopathological markers and the expression of cerbB2, p53 and Ki67. Reduced expression of nuclear BRCA1 protein was found to exert an independent favorable impact on both the overall and relapse-free (RF) survival of the patients (p = 0.019 and p = 0.043, respectively). Cytoplasmic BRCA1 was associated with none of the classic histomorphological indices, except from the lymph node metastasis, with which its relation was found to be inverse (p = 0.05), prolonging the RF survival of the patients (p = 0.05). Our findings suggest that BRCA1 protein depicts different prognostic significance, according to its subcellular distribution. Nuclear detection of the protein was associated with a worse prognosis, while the cytoplasmic one was related to fewer recurrences as a result of fewer lymph node metastases. © 2014 Elsevier GmbH.
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