TY - JOUR
TI - Validation of a real-time quantitative polymerase chain reaction method for the quantification of 3 Survivin transcripts and evaluation in breast cancer tissues
AU - Pavlidou, A.
AU - Kroupis, C.
AU - Goutas, N.
AU - Dalamaga, M.
AU - Dimas, K.
JO - Clinical Breast Cancer
PY - 2014
VL - 14
TODO - 2
SP - 122-131
PB - Cancer Information Group, LP
SN - 1526-8209, 1938-0666
TODO - 10.1016/j.clbc.2013.10.012
TODO - estrogen receptor;  messenger RNA;  progesterone receptor;  survivin;  survivin 2b;  survivin deltaex3;  unclassified drug, agar gel electrophoresis;  article;  breast cancer;  cancer grading;  correlation analysis;  disease association;  human;  human tissue;  lymph node metastasis;  protein expression;  quantitative assay;  real time polymerase chain reaction;  real time quantitative polymerase chain reaction;  sensitivity and specificity;  tumor volume;  validation study, Apoptosis;  Dual hybridization probes;  Prognostic biomarkers;  qPCR;  Survivin splice variants, Breast Neoplasms;  Female;  Gene Expression Regulation, Neoplastic;  Humans;  Inhibitor of Apoptosis Proteins;  Neoplasm Grading;  Neoplasm Invasiveness;  Prognosis;  Protein Isoforms;  RNA Splicing;  Survival Rate
TODO - Background Survivin is a novel antiapoptotic gene, which is a member of the inhibitor of apoptosis protein (IAP) family. Recently, 3 splice variants of this gene were cloned and characterized. This study aimed to validate a sensitive and specific method for the detection of survivin variants in breast cancer. Methods Real-time quantitative polymerase chain reaction (qPCR) was performed on the cDNA with a reverse primer specific for each splice variant and a pair of common hybridization probes. Results The expression of wild-type survivin was significantly correlated with survivin-2b, survivin-ΔEx3, and the ratio of survivin-ΔEx3 to wild-type survivin (P <.001). The ratio of survivin-2b to wild-type survivin was strongly associated with the ratio of survivin-ΔEx3 to wild-type survivin (P <.001). There was a strong positive association between the grade of the tumor and survivin-2b mRNA, survivin-ΔEx3 mRNA, and the ratio of survivin-ΔEx3 to wild-type survivin mRNA (P <.05). The ratio of survivin-2b to wild-type survivin was significantly associated with the presence of estrogen receptors (P =.05). Conclusion Our validated data suggest that survivin isoforms may be related to clinicopathological features and could be used as molecular prognostic tools or as new therapy targets. © 2014 Elsevier Inc. All rights reserved.
ER -