TY - JOUR
TI - Rates of glucose uptake in adipose tissue and muscle in vivo after a mixed meal in women with morbid obesity
AU - Mitrou, P.
AU - Boutati, E.
AU - Lambadiari, V.
AU - Maratou, E.
AU - Papakonstantinou, A.
AU - Komesidou, V.
AU - Sidossis, L.
AU - Tountas, N.
AU - Katsilambros, N.
AU - Economopoulos, T.
AU - Raptis, S.A.
AU - Dimitriadis, G.
JO - JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM
PY - 2009
VL - 94
TODO - 8
SP - 2958-2961
PB - Japan Endocrine Society
SN - 0021-972X
TODO - 10.1210/jc.2008-2297
TODO - glucose;  insulin, adipose tissue;  adult;  article;  clinical article;  controlled study;  diet;  female;  food intake;  glucose blood level;  glucose metabolism;  glucose transport;  hormone blood level;  human;  human tissue;  in vivo study;  insulin blood level;  insulin resistance;  morbidity;  obesity;  priority journal;  test meal
TODO - Background and Aims: Although whole-body insulin resistance in obesity is established, information on insulin action in peripheral tissues, especially adipose tissue (AD), is limited. This study was undertaken in morbid obesity to investigate insulin action on glucose disposal in AD and muscle (M). Subjects and Methods: A meal was given to 30 obese (age 34 ± 1 yr, body mass index 47 ± 1 kg/m2) and 10 nonobese women (age 39 ± 4 yr, body mass index 23 ± 1 kg/m2). Samples for glucose and insulin were taken for 360 min from veins draining the abdominal subcutaneous AD and forearm muscles and from the radial artery. Blood flow (BF) was measured in AD (133Xe) and M (plethysmography). Results: The area under the curve divided by time (AUC0-360min/360min) in obese vs. nonobese was as follows: 1) arterial glucose was similar 6.04 ± 0.2 vs. 5.67 ± 0.1 mM), but insulin was increased (65.5 ± 6.6 vs. 28.7 ± 1.7 mU/liter, P = 0.0004); 2) BF was decreased (3 ± 0.2 vs. 4.4 ± 0.3 ml/min per 100 ml tissue in M, P = 0.002 and 1.8 ± 0.1 vs. 3.7 ± 0.3 ml/min per 100 ml tissue in AD, P < 0.0001); 3) glucose uptake was decreased (0.9 ± 0.1 vs. 2.3 ± 0.4 μmol/min per 100 ml tissue in M, P = 0.002 and 0.45 ± 0.1 vs. 1.1 ± 0.17 μmol/min per 100 ml tissue in AD, P = 0.01); 4) fractional glucose extraction was decreased in M (5 ± 1 vs. 9 ± 1%, P = 0.03), but was similar in AD (3 ± 1 vs. 3.6 ± 1.4%); 5) glucose uptake (per total fat mass) was increased (0.275 ± 0.04 vs. 0.12 ± 0.02 mmol/min, P = 0.027). Conclusion: In morbid obesity, the sensitivity of glucose metabolism to insulin is impaired in M, due to defects in insulin-stimulated glucose use and decreased BF, and in AD, at least in part, due to decreased BF. However, increased total fat mass provides a sink for the excess of glucose and compensates for insulin resistance. Copyright © 2009 by The Endocrine Society.
ER -