TY - JOUR
TI - Isotretinoin therapy induces DNA oxidative damage
AU - Georgala, S
AU - Papassotiriou, L
AU - Georgala, C
AU - Demetriou, E and
AU - Schulpis, KH
JO - Clinical Chemistry and Laboratory Medicine (CCLM)
PY - 2005
VL - 43
TODO - 11
SP - 1178-1182
PB - Walter de Gruyter GmbH
SN - null
TODO - 10.1515/CCLM.2005.204
TODO - cystic acne; DNA damage; free radicals; 8-hydroxy-2-desoxyguanosine
(8-OhdG); isotretinoin
TODO - Background: Isotretinoin (Iso) is currently indicated for the treatment
of cystic acne (CA) and is related to marked teratogenicity.
Aim: The aim of the study was to evaluate the relationship between total
antioxidant status (TAS) and a serum marker of DNA oxidative damage,
8-hydroxy-2-desoxyguanosine (8-OHdG), in patients on Iso treatment.
Patients and methods: Patients with CA (n=18) were evaluated before and
45 days after Iso (0.5 mg/kg per day) treatment and non-diseased
controls (n = 22) were tested only once. Plasma TAS levels and 8-OHdG
were measured spectrophotometrically and with an immunoassay,
respectively. Liver biochemical parameters and muscle enzymes were
measured on a blood chemistry analyzer.
Results: TAS levels were significantly (p < 0.0001) lower in patients
before treatment (921 +/- 124 mu mol/L) compared with those after
treatment (1335 +/- 93 mu mol/L) and in controls (1536 +/- 126 mu
mol/L). In contrast, 8-OHdG serum levels were two-fold higher in
patients after treatment (0.21 +/- 0.03 ng/mL) than before treatment
(0.11 +/- 0.02 ng/mL) and three-fold higher than in controls (0.07 +/-
0.01 ng/mL;p < 0.0001). Negative correlations were found between TAS and
8-OHdG (r=-0.754, p < 0.0001) in patients before therapy and positive
correlations were found between creatine kinase (CK) and 8-OHdG
(r=0.488, p < 0.001) and liver enzymes after Iso treatment.
Conclusions: High serum levels of 8-OHdG in patients on Iso therapy may
be due to a direct effect of Iso on liver, muscle and skin epidermal
cells. Regular evaluation of 8-OHdG in sera of patients, especially of
women of reproductive age, on Iso treatment could be a sensitive
follow-up biomarker of DNA oxidation.
ER -