TY - JOUR
TI - Simultaneous single-tube PCR assay for the detection of Neisseria
meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae
AU - Tzanakaki, G
AU - Tsopanomichalou, M
AU - Kesanopoulos, K
AU - Matzourani,
AU - R
AU - Sioumala, M
AU - Tabaki, A
AU - Kremastinou, J
JO - Clinical Microbiology and Infection
PY - 2005
VL - 11
TODO - 5
SP - 386-390
PB - Elsevier Sci Ltd, Exeter, United Kingdom
SN - 1198-743X
TODO - 10.1111/j.1469-0691.2005.01109.x
TODO - diagnosis; Haemophilus influenzae; meningitis; Neisseria meningitidis;
PCR; Streptococcus pneumoniae
TODO - Rapid, accurate and inexpensive diagnosis of bacterial meningitis is
critical for patient management. This study describes the development
and evaluation of a multiplex PCR assay for the detection of Neisseria
meningitidis, Streptococcus pneumoniae and Haemophilus influenzae type
b, which globally account for 90% of cases of bacterial meningitis. The
single-tube assay, based on the ctrA, ply and bex targets, respectively,
enabled detection of 5-10 pg DNA. When the assay was tested with
clinical samples (n = 425), its sensitivity for the three targets was
93.9%, 92.3% and 88%, respectively, while the overall specificity and
positive predictive value of the assay was 100%. The negative
predictive value was 99.1-99.5%. The methodology permits rapid and
accurate detection of the three main pathogens that cause bacterial
meningitis.
ER -