TY - JOUR
TI - Experimental isolation and transplantation of hepatocytes with the use
of antibody against interleukin-2 receptor (Daclizumab) as
immunosuppressive agent
AU - Tsiolis, I
AU - Papalois, A
AU - Loukopoulos, I
AU - Gravvanis, A and
AU - Lykoudis, E
AU - Theodossopoulou, E
AU - Chairakakis, A and
AU - Dimitroulopoulos, D
AU - Sfiniadakis, I
AU - Vassiliou, I
AU - Felekouras,
AU - E
AU - Dedeilias, P
AU - Kontogiorgi, M
AU - Papadimitrou, L and
AU - Papadimitriou, I
JO - Transplantation Proceedings
PY - 2005
VL - 37
TODO - 4
SP - 1929-1930
PB - EXCERPTA MEDICA INC-ELSEVIER SCIENCE INC
SN - 0041-1345
TODO - 10.1016/j.transproceed.2005.02.097
TODO - null
TODO - Introduction. Daclizumab (Dmab) is a genetically engineered humanized
IgG1 monoclonal antibody that binds to the a chain of the interleukin-2
receptor (Tac, CD25, p55) expressed on activated human T lymphocytes.
Dmab has been used in a clinical protocol of islet transplantation with
satisfactory results. The aim of the present study was to evaluate the
use of an antibody against the interleukin-2 receptor (Dmab) as an
immunosuppressive agent in an experimental model of hepatocyte
allotransplantation (allo-Tx) in rats with fulminant hepatic failure
(FHF).
Materials and methods. Six Wistar rats were used as donors and 48 Lewis
rats as recipients: four groups of 12 animals each with induction of FHF
and 24 hour later hepatocyte Tx-group A: no treatment; group B:
cyclosporin (20 mg/kg days 0 to 5 and 10 mg/kg days 6 to 15); group C:
Dmab (0.05 mg day of Tx and 0.05 mg day 7); and group D: Dmab and
cyclosporine. Hepatocytes were transplanted intrasplenically. Animals
were followed for 15 days.
Results. Statistical analysis showed better survival among groups C
(83%, MST = 13) and D (92%, MST = 14.25) compared to groups A (max 72,
MST = 1.5) or B (50%, MST = 9). Survival in group D was better but not
significantly than group C. Biochemical evaluation and histology
confirmed satisfactory function and engraftment, respectively.
Conclusion. This experimental model showed the safe, effective use of
Dmab.
ER -