TY - JOUR
TI - Relationship of BAL and lung tissue CD4+ and CD8+ T lymphocytes, and
their ratio in idiopathic pulmonary fibrosis
AU - Papiris, SA
AU - Kollintza, A
AU - Kitsanta, P
AU - Kapotsis, G and
AU - Karatza, A
AU - Milic-Emili, J
AU - Roussos, C
AU - Daniil, Z
JO - Chest
PY - 2005
VL - 128
TODO - 4
SP - 2971-2977
PB - Elsevier
SN - 0012-3692, 1931-3543
TODO - 10.1016/S0012-3692(15)52722-0
TODO - BAL; CD4+; CD8+; flow cytometry; idiopathic pulmonary fibrosis;
immunohistochemistry; inflammation T lymphocytes
TODO - Background: Surgical biopsy specimens have shown that T lymphocytes
(TLs) infiltrate lung parenchyma in patients with idiopathic pulmonary
fibrosis (IPF) and might play a pathogenetic role. BAIL, a far less
invasive technique, has also been used for the investigation of IPF
pathogenesis. However, controversy exists whether the BAL fluid cellular
profile reflects the cellular composition of the lung parenchyma.
Study objective: To compare infiltrating TLs subpopulations (CD4+, CD8+,
and CD4+/CD8+ ratio) in lung tissue and BAL fluid.
Patients and methods: Immunohistochemistry was performed according to
the streptavidin-biotin method on the surgical biopsy specimens 4 12
untreated patients with IPF. The number of CD3+, CD4+, and CD8+ TLs was
determined by observer-interactive computerized image analysis (SAMBA
microscopic image processor; Meylan, France). In BAL fluid, the same TLs
subpopulations were evaluated by flow cytometry.
Results: In lung tissue, CD3+ TLs accounted for a mean (+/- SEM) of 28.8
+/- 7% of total cells, CD4+ TLs accounted for 14.5 +/- 4% of total
cells (50.1 +/- 4% of CD3+ TLs), and CD8+ TLs accounted for 13.8 +/-
4% of total cells (47.4 +/- 4% of CD3+ TLs). In BAL fluid, lymphocytes
accounted for 9.8 +/- 2.5% of total cells, CD4+ TLs accounted for 51.8
+/- 4% of CD3+ TLs, and CD8+ TLs accounted for 42.2 +/- 4% of CD3+
TLs. Tissue CD4+ and CD8+ TLs (expressed as a percentage of CD3+ TLs)
correlated significantly with the number of CD4+ and CD8+ TLs in BAIL
fluid (r = 0.846 and p = 0.001 vs; r = 0.692 and p = 0.013,
respectively). A significant positive correlation was also found between
the mean CD4+/CD8+ ratio found in tissue and BAL fluid (1.05 +/- 0.21
and 1.5 +/- 0.27, respectively; r = 0.832; p = 0.01).
Conclusion: The results suggest that in patients with IPF, the TL
subpopulations in BAL fluid reflect the pattern of lymphocytic
infiltration in pulmonary parenchyma.
ER -