TY - JOUR
TI - Evaluation of the clinical sensitivity for the quantification of human
immunodeficiency virus type 1 RNA in plasma: Comparison of the new COBAS
TaqMan HIV-1 with three current HIV-RNA assays-LCx HIV RNA quantitative,
VERSANT HIV-1 RNA 3.0 (bDNA) and COBAS AMPLICOR HIV-1 Monitor v1.5
AU - Katsoulidou, A
AU - Petrodaskalaki, M
AU - Sypsa, V
AU - Papachristou, E
AU - and Anastassopoulou, CG
AU - Gargalianos, P
AU - Karafoulidou, A and
AU - Lazanas, M
AU - Kordossis, T
AU - Andoniadou, A
AU - Hatzakis, A
JO - Journal of Virological Methods
PY - 2006
VL - 131
TODO - 2
SP - 168-174
PB - ELSEVIER SCIENCE BV
SN - 0166-0934
TODO - 10.1016/j.jviromet.2005.07.014
TODO - HIV-1 RNA; viral load; cobas TaqMan; LCx; branched DNA; COBAS amplicor
monitor v1.5
TODO - The COBAS TaqMan HIV-1 test (Roche Diagnostics) was compared with the
LCx HIV RNA quantitative assay (Abbott Laboratories), the Versant HIV-1
RNA 3.0 (bDNA) assay (Bayer) and the COBAS Amplicor HIV-1 Monitor v1.5
test (Roche Diagnostics), using plasma samples of various viral load
levels from HIV-1-infected individuals. In the comparison of TaqMan with
LCx, TaqMan identified as positive 77.5% of the 240 samples versus
72.1% identified by LCx assay, while their overall agreement was 94.6%
and the quantitative results of samples that were positive by both
methods were strongly correlated (r = 0.91). Similarly, in the
comparison of TaqMan with bDNA 3.0, both methods identified 76.3% of
the 177 samples as positive, while their overall agreement was 95.5%
and the quantitative results of samples that were positive by both
methods were strongly correlated (r = 0.95). Finally, in the comparison
of TaqMan with Monitor v 1.5, TaqMan identified 79.5% of the 156
samples as positive versus 80.1% identified by Monitor v 1.5, while
their overall agreement was 95.5% and the quantitative results of
samples that were positive by both methods were strongly correlated (r =
0.96). In conclusion, the new COBAS TaqMan HIV-1 test showed excellent
agreement with other widely used commercially available tests for the
quantitation of HIV-1 viral load. (c) 2005 Elsevier B.V. All rights
reserved.
ER -