TY - JOUR
TI - Effect of serotonin receptor 2 blockage on liver regeneration after
partial hepatectomy in the rat liver
AU - Papadimas, GK
AU - Tzirogiannis, KN
AU - Panoutsopoulos, GI
AU - Demonakou,
AU - MD
AU - Skaltsas, SD
AU - Hereti, RI
AU - Papadopoulou-Daifoti, Z and
AU - Mykoniatis, MG
JO - Liver International
PY - 2006
VL - 26
TODO - 3
SP - 352-361
PB - Wiley
SN - 1478-3223, 1478-3231
TODO - 10.1111/j.1478-3231.2005.01230.x
TODO - ketanserin; liver regeneration; partial hepatectomy; serotonin
TODO - The effect of serotonin receptor 2 blockade (5-HT2) on liver
regeneration after 30-34% and 60-70% partial hepatectomy in the rat
liver was investigated. Materials and methods: Male Wistar rats were
subjected to 60-70% (group I) and 30-34% (group II) partial
hepatectomy. Serotonin receptor 2 blockade was exerted by
intraperitoneal administration of ketanserin at different doses and time
points after partial hepatectomy. The rats of all groups were killed at
different time points until 96 h after partial hepatectomy. The rate of
liver regeneration was evaluated by the mitotic index in hematoxylin and
eosin sections, the immunochemical detection of Ki67 and proliferating
cell nuclear antigens, the rate of [H-3]-thymidine incorporation into
hepatic DNA and liver thymidine kinase enzymatic activity. Results:
Liver regeneration peaked at 24 and 32 h after partial hepatectomy in
60-70% hepatectomized rats. In 30-34% hepatectomized rats liver
regeneration peaked at 60 h, whereas low rates of regenerative activity
were observed between 24 and 72 h after partial hepatectomy. Ketanserin
administration arrested liver regeneration only when administered at 16
h after 60-70% partial hepatectomy. Ketanserin also abrogated the
observed peak of regenerative activity at 60 h in 30-34% hepatectomized
rats when administered at 52 h after partial hepatectomy. All indices of
liver regeneration were affected by ketanserin administration.
Conclusions: Serotonin receptor 2 blockade can arrest liver regeneration
only when administered close to G1/S transition point, and that while
serotonin may be a cofactor for DNA synthesis, it does not play a role
in initiation of liver regeneration.
ER -