TY - JOUR
TI - Increased glycosaminoglycans in the urine of asthmatic children on
inhaled corticosteroids
AU - Priftis, KN
AU - Loukopoulou, S
AU - Magkou, C
AU - Sitaras, NM
JO - Pediatric Allergy and Immunology
PY - 2006
VL - 17
TODO - 3
SP - 194-198
PB - Wiley
SN - 0905-6157, 1399-3038
TODO - 10.1111/j.1399-3038.2006.00387.x
TODO - asthma; children; inhaled corticosteroids; remodeling;
glycosaminoglycans
TODO - Increased extracellular matrix (ECM) deposition in the airway wall
contributes to the airway remodeling in asthmatics. Glycosaminoglycans
(GAGs) are polysaccharides attached to a protein core in order to form
proteoglycans, a component of the ECM. In this study, we investigated
the possible influence of long-term treatment with inhaled
corticosteroids (ICS) on urinary GAGs levels of asthmatic children.
Seventy asthmatic children (41 boys), aged 6.8-12.5 yr, participated in
the study. About 44 were treated with inhaled budesonide via turbuhaler
for 2-35 months (median 12 months) and 26 were on relief medications.
About 30 healthy controls were also studied. GAGs were precipitated from
early morning urine samples, collected, isolated and quantified using
uronic acid-carbazole reaction and expressed as uronic acid (UA) in mu
g/g/Cr-u/m(2). Urinary GAGs values did not differ significantly between
controls and asthmatics but significant differences were found between
children on ICS and asthmatics on relief medications (p < 0.001). There
was a positive correlation between the daily dose of inhaled budesonide
and the urinary GAGs values (r = 0.32, p = 0.037) whereas a threshold
distinguishing ‘low’ vs. ‘high’ doses of ICS was found to be at 300 mu
g/m(2) per day with a significant difference in urinary GAGs secretion
(p = 0.006). Our data show that urinary GAGs secretion is reduced in
asthmatic children that used only relief medication but it is increased
in those on long-term treatment with ICS. A dose dependent effect of ICS
was also detected.
ER -