TY - JOUR
TI - Platelet-activating factor (PAF) increase intracellular lipid
accumulation by increasing both LDL and scavenger receptors in human
mesangial cells
AU - Fragopoulou, Elizabeth
AU - Iatrou, Christos
AU - Antonopoulou, Smaragdi
AU - and Ruan, Xiong-Zhong
AU - Fernando, Ray L.
AU - Powis, Stephen H. and
AU - Moorhead, John F.
AU - Varghese, Zac
JO - Journal of Laboratory and Clinical Medicine
PY - 2006
VL - 147
TODO - 6
SP - 281-289
PB - MOSBY-ELSEVIER
SN - 0022-2143
TODO - 10.1016/j.lab.2006.01.004
TODO - null
TODO - Intra- and extracellular lipid accumulation and the production of
inflammatory mediators by renal and accessory cells may play an
important role in the initiation and progression of these lesions.
Platelet activating factor (PAF) is a biologically active phospholipid
that is produced by various cells upon activation by different stimuli.
It has been suggested that PAF plays a role in atherogenesis, and
several studies indicated its participation in the pathogenesis of renal
diseases. The aim of this study is to investigate the role of PAF on
intracellular lipid accumulation and gene regulation of lipoprotein
receptors in human mesangial cells (HMCs). A human mesangial cell line
(HMC) was used to study the effects of PAF on foam cell formation by Oil
red O staining and on the expression of LDLr, SR-Al, and PAF-R mRNA
using RT-PCR. Native LDL caused foam cell formation in HMC in the
presence of PAR PAF enhanced LDLr expression and overrode LDL receptor
suppression induced by a high concentration of LDL. Moreover, it
enhanced SR-Al expression. PAF also caused increase in PAF-R expression.
The above data suggest that PAF enhances its own receptor expression and
then increases lipid accumulation by dysregulating LDL receptor
regulation and inducing scavenger receptor expression in HMCs. These
results suggest that PAF has a potential role in lipid mediated renal
injury.
ER -