TY - JOUR
TI - Liver regeneration: Immunohistochemichal study of intrinsic hepatic innervation after partial hepatectomy in rats
AU - Kandilis, A.N.
AU - Koskinas, J.
AU - Vlachos, I.
AU - Skaltsas, S.
AU - Karandrea, D.
AU - Karakitsos, P.
AU - Pantopoulou, A.
AU - Palaiologou, M.
AU - Nikiteas, N.
AU - Tiniakos, D.G.
AU - Perrea, D.N.
JO - BMC Gastroenterology
PY - 2014
VL - 14
TODO - 1
SP - null
PB - BioMed Central Ltd.
SN - 1471-230X
TODO - 10.1186/s12876-014-0202-1
TODO - cell protein;  neuromodulin;  protein gene product 9.5;  unclassified drug;  biological marker;  neuromodulin;  PGP9.5 protein, rat;  ubiquitin thiolesterase, animal experiment;  animal tissue;  Article;  controlled study;  immunohistochemistry;  innervation;  liver innervation;  liver regeneration;  male;  morphometrics;  nerve fiber density;  nerve fiber regeneration;  nerve sprouting;  nervous system parameters;  nonhuman;  partial hepatectomy;  portal vein;  postoperative period;  rat;  tissue section;  animal;  chemistry;  immunohistochemistry;  liver;  liver regeneration;  liver resection;  nerve fiber;  nerve regeneration;  physiology;  Wistar rat, Animals;  Axons;  Biomarkers;  GAP-43 Protein;  Hepatectomy;  Immunohistochemistry;  Liver;  Liver Regeneration;  Male;  Nerve Fibers;  Nerve Regeneration;  Rats, Wistar;  Ubiquitin Thiolesterase
TODO - Background: We examined the intrinsic hepatic innervation after partial hepatectomy (PH) in rats and the presence and pattern of neural sprouting in regenerating liver. Methods: Male Wistar rats (age 9-13 weeks-w, weight 204-356 g), were submitted to two-thirds PH. Rats were sacrificed at postoperative days (d) 1, 3, 5, 7, at 2 and 4 w, and at 3 and 6 months (m) (6-7 animals/group, control group n = 4). Immunohistochemistry for the pan-neural marker protein gene product 9.5 (PGP9.5) and growth-associated protein 43 (GAP-43), a marker of regenerating nerve axons, was performed on tissue sections from the R1 lobe of the regenerating liver. Portal tracts (PTs) with immunoreactive fibers were counted in each section and computer-assisted morphometric analysis (Image Pro Plus) was used to measure nerve fiber density (number of immuno-positive nerve fibers/mm2 (40x)). Results: Immunoreactivity for PGP9.5 was positive in all groups. The number of PGP9.5 (+) nerve fibers decreased from 0.32 +/- 0.12 (control group) to 0.18 +/- 0.09 (1d post-PH group), and gradually increased reaching pre-PH levels at 6 m (0.3 +/- 0.01). In contrast, immunoreactivity for GAP-43 was observed at 5d post-PH, and GAP-43 (+) PTs percentage increased thereafter with a peak at 3 m post-PH. GAP-43 (+) nerve fiber density increased gradually from 5d (0.05 +/- 0.06) with a peak at 3 m post-PH (0.21 +/- 0.027). At 6 m post-PH, immunoreactivity for GAP-43 was not detectable. Conclusions: Following PH in rats: 1) nerve fiber density in portal tracts decreases temporarily, and 2) neural sprouting in the regenerating liver lobes starts at 5d, reaches peak levels at 3 m and disappears at 6 m post-PH, indicating that the increase in hepatic mass after PH provides an adequate stimulus for the sprouting process.
ER -