TY - JOUR
TI - Differential Expression of Collagen Type V and XI α-1 in Human Ascending Thoracic Aortic Aneurysms
AU - Toumpoulis, I.K.
AU - Oxford, J.T.
AU - Cowan, D.B.
AU - Anagnostopoulos, C.E.
AU - Rokkas, C.K.
AU - Chamogeorgakis, T.P.
AU - Angouras, D.C.
AU - Shemin, R.J.
AU - Navab, M.
AU - Ericsson, M.
AU - Federman, M.
AU - Levitsky, S.
AU - McCully, J.D.
JO - The Annals of Thoracic Surgery
PY - 2009
VL - 88
TODO - 2
SP - 506-513
PB - 
SN - 0003-4975, 1552-6259
TODO - 10.1016/j.athoracsur.2009.04.030
TODO - collagen type 1;  collagen type 11;  collagen type 3;  collagen type 5;  fibrillar collagen;  messenger RNA, article;  ascending aorta;  clinical article;  controlled study;  elective surgery;  electron microscopy;  extracellular matrix;  gene expression;  gene mutation;  histopathology;  human;  human tissue;  immunofluorescence test;  priority journal;  protein blood level;  quantitative analysis;  real time polymerase chain reaction;  reverse transcription polymerase chain reaction;  thoracic aorta aneurysm;  tissue section;  Western blotting, Aortic Aneurysm, Thoracic;  Collagen Type V;  Collagen Type XI;  Disease Progression;  Extracellular Matrix;  Humans;  Immunohistochemistry;  Proteins;  Reverse Transcriptase Polymerase Chain Reaction;  RNA, Messenger
TODO - Background: The molecular mechanisms leading to ascending thoracic aortic aneurysms (ATAAs) remain unknown. We hypothesized that alterations in expression levels of specific fibrillar collagens occur during the aneurysmal process. Methods: Surgical samples from ascending aortas from patients with degenerative ATAAs were subdivided by aneurysm diameter: small, 5 to 6 cm; medium, 6 to 7 cm; and large, greater than 7 cm; and compared with nonaneurysmal aortas (mean diameter, 2.3 cm). Results: Histology, immunofluorescence, and electron microscopy demonstrated greater disorganization of extracellular matrix constituents in ATAAs as compared with control with an increase in collagen α1(XI) within regions of cystic medial degenerative lesions. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) showed collagens type V and α1(XI) were significantly and linearly increased in ATAAs as compared with control (p < 0.001). There was no change in the messenger ribonucleic acid (mRNA) expression levels of collagens type I and III. Western blot analysis showed collagens type I and III were significantly decreased and collagens α1(XI) and V were significantly increased and were linearly correlated with the size of the aneurysm (p < 0.001 for both). Conclusions: These results demonstrate that increased collagen α1(XI) and collagen V mRNA and protein levels are linearly correlated with the size of the aneurysm and provide a potential mechanism for the generation and progression of aneurysmal enlargement. © 2009 The Society of Thoracic Surgeons.
ER -