TY - JOUR
TI - Monocytes as a site of production of soluble triggering receptor
expressed on myeloid cells-1 (sTREM-1) in the septic host
AU - Plachouras, Diamantis
AU - Routsi, Christina
AU - Giamarellos-Bourboulis,
AU - Evangelos J.
AU - Spyridaki, Ekaterini
AU - Andrianakis, Ilias and
AU - Metzelopoulos, Spyridon
AU - Tsaganos, Thomas
AU - Floros, Ioannis and
AU - Douzinas, Emmanuel E.
AU - Armaganidis, Apostolos
AU - Roussos, Charis and
AU - Giamarellou, Helen
JO - Scandinavian Journal of Infectious Diseases
PY - 2006
VL - 38
TODO - 10
SP - 909-915
PB - Informa Healthcare
SN - 0036-5548, 1651-1980
TODO - 10.1080/00365540600786523
TODO - null
TODO - The role of blood monocytes in the secretion of soluble triggering
receptor expressed on myeloiod cells (sTREM-1) was studied in 90
patients with septic syndrome due to ventilator-associated pneumonia.
Blood monocytes were isolated on 7 consecutive d after initiation of
symptoms. Monocytes were incubated in the absence or presence of LPS and
concentrations of sTREM-1 and TNF alpha in cell supernatants and serum
were estimated by an enzyme-immunoassay. sTREM-1 and TNF alpha were
consistently present at detectable levels in the cell supernatants. LPS
induced increased levels of TNFa but not of sTREM-1. Supernatants
recovered from monocytes on d 1 showed levels of sTREM-1 higher than
those recovered on any of the following 6 d (p < 0.05); these levels
were higher in non-survivors than in survivors. Supernatants recovered
from monocytes on d 1 of patients with severe sepsis had elevated
concentrations of sTREM-1 compared to patients with septic shock and
similar to patients with sepsis. A negative correlation was found
between levels of sTREM-1 in the cell supernatants and the percentage of
apoptotic monocytes. In essence, the above results suggest that
monocytes contribute to the production of sTREM-1 in the event of septic
syndrome.
ER -