TY - JOUR
TI - Maternal-neonatal 8-hydroxy-deoxyguanosine serum concentrations as an
index of DNA oxidation in association with the mode of labour and
delivery
AU - Schulpis, Kleopatra H.
AU - Lazaropoulou, Christina
AU - Vlachos, George
AU - D.
AU - Partsinevelos, George A.
AU - Michalakakou, Kelly
AU - Gavrili,
AU - Stavroula
AU - Gounaris, Antonia
AU - Antsaklis, Aris
AU - Papassotiriou,
AU - Ioannis
JO - Acta Obstetricia et Gynecologica Scandinavica
PY - 2007
VL - 86
TODO - 3
SP - 320-326
PB - Wiley
SN - 0001-6349, 1600-0412
TODO - 10.1080/00016340601181706
TODO - 8-hydroxy-deoxyguanosine; labour; neonates; oxidative stress
TODO - Aim. To investigate the effect of the mode of labour and delivery on the
total antioxidant status (TAS), and the biomarker of DNA oxidation,
8-hydroxy-deoxyguanosine (8-OHdG) serum levels, in mothers and their
newborns. Subjects and methods. Some 106 women with normal pregnancy and
normal blood biochemical parameters were divided into 4 groups: Group A
( n = 28) with normal labour and vaginal delivery ( VG), Group B ( n =
25) with scheduled cesarean section ( CS), Group C ( n = 26) with
‘emergency’ CS, and Group D ( n = 27) with prolonged labour + VG. Blood
was obtained fr? m the mothers at the beginning of labour, and
immediately after delivery ( pre- and post-delivery), as well as from
the umbilical cord ( CB). TAS, 8-OHdG and creatine kinase (CK) were
measured in the sera with appropriate methodology. Results. TAS levels
were almost similar in all the groups pre- delivery, and in CB
irrespective of the mode of labour and delivery, and remarkably
decreased in Groups C and D post-delivery. 8-OHdG levels in Group C (
0.94 +/- 0.08 ng/ml) and Group D (0.98 +/- 0.08 ng/ml) were
significantly higher than those in Group A (0.26 +/- 0.01 ng/ml, p <
0.001) and Group B (0.28 +/- 0.07 ng/ml, p < 0.001) post-delivery.
8-OHdG levels were low in CB, independent of the mode of labour. CK
positively correlated with 8-OHdG ( r = 0.48, p < 0.001), the latter
negatively correlated with TAS ( r = - 0.53, p < 0.01). Conclusions. The
lowest TAS and the highest 8-OHdG levels were found in Groups C and D
post-delivery, probably due to the long-term participation of the
mothers’ skeletal and uterus muscles, whereas 8-OHdG levels were low in
CB irrespective of the mode of delivery, possibly as a consequence of
the antioxidant action of the placenta and/or the low lipid levels in
the serum of the umbilical cord.
ER -