TY - JOUR
TI - Anti proliferative effect of adiponectin on MCF7 breast cancer cells: a
potential hormonal link between obesity and cancer
AU - Arditi, J. D.
AU - Venihaki, M.
AU - Karalis, K. P.
AU - Chrousos, G. P.
JO - Hormone and Metabolic Research
PY - 2007
VL - 39
TODO - 1
SP - 9-13
PB - Georg Thieme Verlag KG
SN - 0018-5043, 1439-4286
TODO - 10.1055/s-2007-956518
TODO - adipokines; tumorigenesis; metabolism
TODO - Adiponectin, a hormone secreted by adipose tissue, circulates at high
concentrations in human plasma. Paradoxically, plasma levels of
adiponectin are approximately 50% lower in obese than in lean subjects.
An association between low plasma levels of adiponectin and higher risk
of developing breast and other cancers was recently reported. Obesity
and overweight have also been associated with increased mortality from
cancer. To test the hypothesis that adiponectin exerts direct anti
proliferative and/or pro-apoptotic effects on cancer cells, we used the
MCF7 human breast adenocarcinoma cell line. The proliferation rate of
the MCF7 cells was measured using the MTT method, while apoptosis was
examined by quantifying the DNA fragmentation using an ELISA assay. In
addition, adiponectin receptor 1 (AdipoR1) and AdipoR2 mRNA expression
was detected using RT-PCR. Adiponectin diminished the proliferation rate
of MCF7 cells; this effect was significant after 48-96 hours of
treatment. The presence of receptor expression suggested that the effect
of adiponectin on cell proliferation was most likely specific and
adiponectin receptor-mediated. Adiponectin induced no apoptosis of MCF7
cells over 48 hours. We conclude that adiponectin inhibits proliferation
but causes no apoptosis of MCF7 breast cancer cells. These data suggest
that adiponectin may represent a direct hormonal link between obesity
and cancer.
ER -