TY - JOUR
TI - Evaluation of claspin as a proliferation marker in human cancer and
normal tissues
AU - Tsimaratou, K.
AU - Kletsas, D.
AU - Kastrinakis, N. G.
AU - Tsantoulis, P.
AU - K.
AU - Evangelou, K.
AU - Sideridou, M.
AU - Liontos, M.
AU - Poulias, I.
AU - and Venere, M.
AU - Salmas, M.
AU - Kittas, C.
AU - Halazonetis, T. D. and
AU - Gorgoulis, V. G.
JO - Iranian Journal of Pathology
PY - 2007
VL - 211
TODO - 3
SP - 331-339
PB - Wiley
SN - null
TODO - 10.1002/path.2095
TODO - cancer; cell proliferation; claspin; immunohistochemistry; monoclonal
antibody
TODO - Claspin is a nuclear protein involved in DNA replication and the DNA
damage response. Its structural and functional properties suggest that
it may represent a potentially useful proliferation marker. To this end,
a monoclonal antibody was generated and the expression of claspin was
investigated in normal fibroblasts and various cancer cell lines, as
well as in tumour and normal tissues from patients with primary
epithelial carcinomas. Immunoblotting analysis confirmed the specificity
of the antibody, while immunohistochemistry demonstrated its
applicability in archival material. In normal cells and tissues, claspin
expression was weak, whereas increased levels were observed in cancer
cell lines and tumour specimens. Claspin staining correlated strongly
with Ki67 staining in both normal (p < 0.001) and tumour tissues (p <
0.001). However, the labelling index (LI) of claspin was consistently
lower than that of Ki67, suggesting that claspin expression may be
limited to a narrower part of the cell cycle. Co-localization assays
with cyclin A and cell synchronization experiments indicated that
claspin expression coincides with the S phase. Interestingly, the
relative increase of the claspin LI in tumour samples compared with
normal tissues was significantly higher (14-fold) than that of the Ki67
LI (five-fold), suggesting that claspin may be a more sensitive marker
of aberrant proliferation. Copyright (c) 2006 Pathological Society of
Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
ER -