TY - JOUR
TI - Tissue concentration of transforming growth factor beta 1 and basic
fibroblast growth factor in skin wounds created with a CO2 laser and
scalpel: A comparative experimental study, using an animal model of skin
resurfacing
AU - Manolis, Evangelos N.
AU - Kaklamanos, Ioannis G.
AU - Spanakis, Nicholas
AU - and Filippou, Dimitrios K.
AU - Panagiotaropoulos, Theophanis and
AU - Tsakris, Athanassios
AU - Siomos, Konstadinos
JO - Wound Repair and Regeneration
PY - 2007
VL - 15
TODO - 2
SP - 252-257
PB - Wiley
SN - 1067-1927, 1524-475X
TODO - 10.1111/j.1524-475X.2007.00212.x
TODO - null
TODO - Although a number of ablative-laser techniques based on CO2 and Er: YAG
laser devices have been successfully developed and used in the clinical
setting, the bio-molecular processes influencing wound healing after
exposure to laser energy are not well elucidated. In this study, we aim
to assess the impact of the mechanism of injury on the secretion of
transforming growth factor beta 1 (TGF-beta 1) and basic fibroblast
growth factor (bFGF) in various stages of wound healing, in wounds
created with a CO2 laser and scalpel. Ten Wistar rats were used to
determine the levels of growth factor proteins TGF-beta 1 and bFGF after
CO2 laser- and scalpel-induced skin injury. Tissue was excised on day 0
for untreated skin (control sites), and on days 1, 10, 30, and 90
following laser and scalpel surgery. Specimens were processed for
histopathological analysis and for determining the concentration of
growth factors by a Western blot technique. The concentration of
TGF-beta 1 increased markedly, at day 1 postinjury, from a baseline of
130 +/- 16 mm(2) (mean surface area of blotted-protein lanes) to 261 +/-
23 mm(2) and 394 +/- 22 mm(2) for laser-inflicted injury and scalpel
wounds, respectively; the latter values were found to differ
significantly (p < 0.001). The concentration of b-FGF on day 10
postinjury differed significantly (p < 0.001) between the laser sites
(553 +/- 45 mm(2)) and the corresponding scalpel sites (418 +/- 41
mm(2)). Laser energy alters local tissue secretion of TGF-beta 1 and
bFGF of skin injuries created with the CO2 laser compared with wounds
created with a scalpel. These differences might have an impact on
various aspects of wound healing of skin injuries created by a laser.
ER -