TY - JOUR
TI - TGF beta RII, BAX, IGFIIR, caspase-5, hMSH3 and hMSH6 alterations are
not associated with microsatellite instability or p53 mutations in
invasive urothelial carcinoma of the urinary bladder
AU - Saetta, Angelica A.
AU - Korkolopoulou, Penelope
AU - Karlou, Maria and
AU - Levidou, Georgia
AU - Goudopoulou, Athina
AU - Thymara, Irene and
AU - Stamatelli, Angeliki
AU - Tzivras, Michalis
AU - Michalopoulos, Nikolaos
AU - V.
AU - Thomas-Tsagli, Euphemia
AU - Patsouris, Efstratios
JO - EMC - Toxicologie-Pathologie
PY - 2007
VL - 39
TODO - 4
SP - 425-432
PB - Elsevier
SN - null
TODO - 10.1080/00313020701444457
TODO - BAY; hMSH3; hMSH6; IGFIIR; TGF-beta RII; urothelial carcinoma; MSI
TODO - Aim: The aim of this study was to determine the potential synchronous
contribution of alterations in TGF-beta RII, BAX, IGFIIR, caspase-5,
hMSH3 and hMSH6 genes to the development and clinical outcome of bladder
cancer, in relation to p53 mutations, microsatellite status and
hMLH1/hMSH2 expression.
Methods: Molecular biology techniques as well as immunohistochemistry
were applied in 69 samples from patients with urothelial carcinoma.
Results: Microsatellite alterations were observed in TGF-beta RII(A)(10)
(16%) and BAX(G)(8) (3%), irrespective of the presence of p53
mutations, but not in IGFIIR(G)(8), caspase-5(A)(10), hMSH3(A)(8) and
hMSH6(C)(8). A statistically significant correlation could be found only
between hMLH1 expression and the presence of microsatellite instability
(Fisher’s exact test, p=0,013). Survival analysis indicated that apart
from grade and T-category, hMLH1 expression was the only parameter
significantly affecting overall survival (p=0.021 in univariate and
p=0.015 in multivariate analysis) and recurrence-free survival (p=0.0463
in univariate and p=0.022 in multivariate analysis).
Conclusions: We conclude that alterations of the examined target genes
of MSI are rare in urinary bladder carcinoma and they are not associated
with microsatellite instability or the presence of p53 mutations.
ER -