TY - JOUR
TI - Experimental Models of Partial Intestinal Obstruction in Young Mice, Establishment, and Evaluation
AU - Georgopoulos, I.
AU - Mavrigiannaki, E.
AU - Stasinopoulou, S.
AU - Renieris, G.
AU - Nikolakis, G.
AU - Chaniotakis, I.
AU - Tiniakos, D.
AU - Papaconstantinou, I.
JO - Journal of Surgical Research
PY - 2020
VL - 252
TODO - null
SP - 206-215
PB - Academic Press Inc.
SN - 0022-4804, 1095-8673
TODO - 10.1016/j.jss.2020.03.007
TODO - animal euthanasia;  animal experiment;  animal model;  animal tissue;  animal welfare;  Article;  dilatation;  experimental model;  female;  histopathology;  hyperplasia;  incision;  intestine loop;  ligation;  long term survival;  male;  mesentery;  mortality rate;  mouse;  mouse model;  muscle hypertrophy;  nonhuman;  priority journal;  small intestine content;  small intestine muscle;  small intestine obstruction;  small intestine wall;  smooth muscle cell;  surgical technique;  survival rate;  survival time;  suture technique;  time of death;  adverse event;  animal;  C57BL mouse;  disease model;  economics;  education;  human;  intestine mucosa;  intestine obstruction;  pathology;  pathophysiology;  randomization;  reproducibility;  small intestine;  surgery, Animals;  Disease Models, Animal;  Female;  Humans;  Intestinal Mucosa;  Intestinal Obstruction;  Intestine, Small;  Ligation;  Male;  Mice;  Mice, Inbred C57BL;  Random Allocation;  Reproducibility of Results;  Suture Techniques
TODO - Background: Partial small bowel obstruction (SBO) is a common, potentially hazardous, surgical entity caused by numerous factors in humans. A number of techniques have been reported as efficient to simulate partial SBO in murine models. However, there is little data concerning their long-term survival. Our study presents a novel technique and evaluates its long-term efficiency compared with other commonly used techniques. Materials and methods: Sixty C57BL/6 mice aged 6 to 8 wk were randomly divided into five intervention groups: ligation, intestinal ring, partial ligation, microclips, and the novel triple suture technique. The ring groups were subdivided into narrow, medium, and wide ring and partial ligation groups were subdivided at 1/3, 1/2, and 2/3 of the lumen. Survival cutoff time was set at 4 wk. Animals were then euthanized and small bowel muscle layer thickness was histopathologically evaluated. Results: None of the animals of the ligation and the ring groups reached the cutoff survival time. The mortality rate of the partial ligation and the microclips groups at the 4-week period were 33.3% and 0%, respectively. However, elimination of the performed intervention was revealed at the time of euthanasia and no alterations of the muscle layer were revealed at histopathology. The “triple suture” group had a survival rate of 90% until euthanasia and the sutures were apparent in all cases. Macroscopic evaluation showed small to mild proximal lumen dilatation in 6 of 10 animals. Histopathological evaluation of the specimens confirmed the partial obstruction. Conclusions: The “triple suture” technique is a new, robust, reliable, and inexpensive technique for experimental long-standing partial SBO, with very low mortality. © 2020 Elsevier Inc.
ER -