TY - JOUR
TI - Expression and prognostic significance of kallikrein-related peptidase 8 protein levels in advanced ovarian cancer by using automated quantitative analysis
AU - Kountourakis, P.
AU - Psyrri, A.
AU - Scorilas, A.
AU - Markakis, S.
AU - Kowaiski, D.
AU - Camp, R.L.
AU - Diamandis, E.P.
AU - Dimopoulos, M.A.
JO - Thrombosis and Haemostasis
PY - 2009
VL - 101
TODO - 3
SP - 541-546
PB - 
SN - 0340-6245
TODO - 10.1160/TH08-01-0052
TODO - neuropsin, adult;  article;  automation;  cancer survival;  follow up;  human;  major clinical study;  ovary cancer;  overall survival;  priority journal;  prognosis;  protein expression;  quantitative analysis;  tissue microarray, Disease-Free Survival;  Female;  Humans;  Immunohistochemistry;  Kallikreins;  Ovarian Neoplasms;  Prognosis;  Protein Array Analysis;  Tissue Array Analysis;  Tumor Markers, Biological
TODO - Kallikrein-related peptidases, a subgroup of the serine protease enzyme family, are considered to be important prognostic biomarkers in cancer. In this study we sought to determine the prognostic value of kallikrein-related peptidase 8 (KLK8,hK8,KLK-8) in ovarian cancer using a novel method of compartmentalised in situ protein analysis.A tissue array composed of 150 advanced stage ovarian cancers, uniformly treated with surgical debulking followed by platinum-paclitaxel combination chemotherapy,was constructed. For the evaluation of kallikrein-related peptidase 8 protein expression, we used an immunofluorescence-based method of automated in situ quantitative protein analysis (AQUA). Mean follow-up time of the cohort was 34.35 months. One hundred twenty-six of 150 cases had sufficient tissue for AQUA analysis.There were significant correlations between tumour mask KLK8 protein expression levels and clinicopathological variables, including grade (p=0.0011), residual disease (p=0.0063) and clinical response to chemotherapy(p=0.0346). In univariate survival analysis there was a significant correlation between KLK8 tumour mask expression and five years progression-free survival, meanwhile it was not associated with five-year overall survival (p =0.0694). Specifically, low KLK8 expression correlated with better outcome (top vs. bottom quartile, p=0.0319). In multivariate survival analysis, adjusting for well-characterised prognostic variables, tumour KLK8 expression level retained its prognostic significance for progression-free survival (95%Cl: 0.341-1.027, p=0.045). The possibility that KLK8 may be a suitable candidate as a diagnostic and prognostic marker warrants further investigation. © 2009 Schattauer GmbH.
ER -