TY - JOUR
TI - Expression of MAGE-A12 in oral squamous cell carcinoma
AU - Mollaoglu, Nur
AU - Vairaktaris, Eleftherios
AU - Nkenke, Emeka and
AU - Neukam, Friedrich W.
AU - Ries, Jutta
JO - Disease Markers
PY - 2008
VL - 24
TODO - 1
SP - 27-32
PB - HINDAWI LTD
SN - 0278-0240, 1875-8630
TODO - 10.1155/2008/359840
TODO - nested RT-PCR; MAGE-A12 gene expression; oral squamous cell carcinoma;
diagnostic; therapy
TODO - Melanoma associated-A antigens (MAGE-A) are silent in normal tissues
except testis. However, they are activated in a variety of different
tumors. Thus, their expression is highly specific to cancer cells.
Reverse transcription-nested polymerase chain reaction (RT-nPCR) is a
highly sensitive technique that has been used successfully for the
detection of MAGE genes in tissue samples. The aim of the study is to
analyze the expression rate of MAGE-A12 in oral squamous cell carcinoma
(OSCC) using a high sensitive RT-nPCR. Total of 57 tissue samples
obtained from patients with OSCC and 20 normal oral mucosal (NOM) probes
of otherwise healthy volunteers were included to this study. No
expression of MAGE-A12 was observed in the non-neoplastic NOM tissues.
MAGE-A12 was expressed in 49.1% of the investigated tumor samples. The
correlation between malignant lesion and MAGE-A12 detection was
significant (p < 0.001). It is concluded that results of this study may
indicate MAGE-A12 as a useful additional diagnostic marker especially
for the early detection of OSCC distinguishing neoplastic transformation
and detection of occult and/or rare disseminated cancer cells. In
addition, MAGE-A12 expression in OSCC may also determine a new
immunotherapeutic target and might be warranted to develop vaccine for
OSCC.
ER -