TY - JOUR
TI - A novel multiple-marker method for the early diagnosis of oral squamous
cell carcinoma
AU - Ries, Jutta
AU - Mollaoglu, Nur
AU - Toyoshima, Takeshi
AU - Vairaktaris,
AU - Eleftherios
AU - Neukam, Friedrich W.
AU - Ponader, Sabine
AU - Nkenke,
AU - Emeka
JO - Disease Markers
PY - 2009
VL - 27
TODO - 2
SP - 75-84
PB - HINDAWI LTD
SN - 0278-0240, 1875-8630
TODO - 10.1155/2009/510124
TODO - Oral squamous cell carcinoma; MAGE-A expression; multiple markers;
RT-PCR; diagnosis
TODO - Objective: Melanoma associated antigens-A (MAGE-A) expression is highly
specific to cancer cells. Thus, they can be the most suitable targets
for the diagnosis of malignancy. The aim of this study was to evaluate
the sensitivity of multiple MAGE-A expression analysis for the diagnosis
of oral squamous cell carcinoma (OSCC).
Methods: Total of 70 OSSC and 20 normal oral mucosal (NOM) samples of
otherwise healthy volunteers were examined for the expression of 10
different single antigens out of 12 different MAGE-A subtypes by highly
sensitive reverse transcriptase polymerase chain reaction (RT-PCR)
methods. The results were correlated to clinicopathological parameters
of tumor samples.
Results: Expression of MAGE-A was restricted to OSCC. The expression
frequency of single antigen was between 10% and 55%. However,
expression rate was increased up to 93% by the elevated number of genes
examined. A significant correlation was found between the expression of
MAGE-A and malignancy (p = 0.0001). In addition, multiple MAGE-A
detection has also correlated to the incidence of lymph node metastasis,
grading and advanced clinical stages.
Conclusions: Analysis of multiple MAGE-A expression is more sensitive
than the analysis of a single MAGE-A for the diagnostic evaluation of
OSCC. Multiple MAGE-A expression analysis may be a very sensitive method
to be used for the diagnosis even in the early stage of OSCC.
ER -