TY - JOUR
TI - Detection of Epithelial Cells by RT-PCR Targeting CEA, CK20, and TEM-8
in Colorectal Carcinoma Patients Using OncoQuick Density Gradient
Centrifugation System
AU - Lagoudianakis, Emmanuel E.
AU - Kataki, Agapi
AU - Manouras, Andreas and
AU - Memos, Nikolaos
AU - Papadima, Artemisia
AU - Derventzi, Anastasia and
AU - Zografos, George
AU - Papadopoulos, Stefanos
AU - Katergiannakis,
AU - Vaggelogiannis
AU - Konstadoulakis, Manoussos M.
JO - Journal of Surgical Research
PY - 2009
VL - 155
TODO - 2
SP - 183-190
PB - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN - 0022-4804, 1095-8673
TODO - 10.1016/j.jss.2007.10.013
TODO - circulating mRNA; CEA; CK20; TEM-8; colon cancer
TODO - Background. The detection of epithelial-specific mRNA correlates well
with the presence of cancer cells in the peripheral blood and provides a
rational explanation for subsequent metastasis.
Material and methods. Forty-two, patients with colorectal cancer and 14
controls were included in our study. Peripheral blood samples were
acquired at 24 h before and 48 It after laparotomy. Tissue samples were
also acquired from the primary lesion. All samples were examined for the
expression profile of CEA, CK20, and TEM-8.
Results. Tissue samples expressed CEA in every specimen, CK20 in 30, and
TEM-8 in 41. CEA and CK20 were not identified in the control blood
samples while TEM-8 was detected in 4. CEA was detected in 17, CK20 in
28 and TEM-8 in 23, of the preoperative blood samples. CEA mRNA
expression in preoperative blood sample and TNM stage were found
independently associated with increased tumor size. Positive CEA, CK20,
and TEM-8 signals were found in 25,25, and 23 of the postoperative blood
samples respectively.
Conclusions. CK20 and CEA are significantly more frequently detected in
colon cancer patients than in healthy controls and can serve as markers.
Cancer cell mRNA is commonly detected in the preoperative and
postoperative peripheral blood samples. Tumor size was independently
associated with the preoperative detection of CEA mRNA. Although TEM-8
mRNA detection in the peripheral blood showed no specificity for cancer
patients or correlation with clinical stage, identification and
validation of genes and proteins implicated in metastatic process needs
to be further investigated. (C) 2009 Elsevier Inc. All rights reserved.
ER -