TY - JOUR
TI - Single Disseminated Tumor Cell Detection in Peripheral Blood Sample of
Patients with Oral Squamous Cell Carcinoma Using MAGE-A4
AU - Mollaoglu, Nur
AU - Vairaktaris, Eleftherios
AU - Nkenke, Emeka and
AU - Neukam, Friedrich W.
AU - Ries, Jutta
JO - LABMEDICINE
PY - 2009
VL - 40
TODO - 11
SP - 665-668
PB - AMER SOC CLINICAL PATHOLOGY
SN - null
TODO - 10.1309/LM1C9Q8DRHYEQHKS
TODO - null
TODO - Background: Present study aimed to investigate the single disseminated.
tumor cells (DTC) in whole peripheral blood samples collected from
patients with oral squamous cell carcinoma (OSCC) for the early
detection of metastatic cells and prognosis by expression analysis of
Melanoma Associated Antigen-A4 (MAGE-A4).
Methods: Eighteen patients with OSCC and 14 otherwise healthy volunteers
were included in this study. The QuantiGene 2:0 RNA Quantification
System was used as a reagent for RNA detection and quantification, and
the results were evaluated by the luminescence reader.
Results: There was no DTC detected by the expression analysis of MAGE-A4
in blood samples collected from patients with OSCC.
Conclusions: Detection of single DTC in peripheral whole blood samples
from OSCC patients may be an effective early detection method when the
suggested steps were included in the method according to the authors’
present experience.
ER -