TY - JOUR
TI - Acute Hyperhomocysteinemia Impairs Endothelium Function in Subjects with
Type 2 Diabetes Mellitus
AU - Doupis, J.
AU - Eleftheriadou, I.
AU - Kokkinos, A.
AU - Perrea, D. and
AU - Pavlatos, S.
AU - Gonis, A.
AU - Katsilambros, N.
AU - Tentolouris, N.
JO - Experimental and Clinical Endocrinology & Diabetes
PY - 2010
VL - 118
TODO - 7
SP - 453-458
PB - JOHANN AMBROSIUS BARTH VERLAG MEDIZINVERLAGE HEIDELBERG GMBH
SN - 0947-7349, 1439-3646
TODO - 10.1055/s-0030-1248290
TODO - homocysteine; type 2 diabetes mellitus; flow-mediated vasodilation;
augmentation index; arterial stiffness
TODO - The objective of the present study was to examine the effect of acute,
methionine-induced hyper-homocysteinemia (HHCY) on endothelial function
and indices of arterial stiffness in subjects with type 2 diabetes
mellitus (T2DM). A total of 30 subjects with T2DM, free of macrovascular
disease were examined in a crossover study. L-methionine (M) (0.1 g/kg)
and water (W) load were given in random order with an interval of about
1 week in between. Endothelial function was assessed by flow-mediated
vasodilation (FMD). Arterial stiffness was assessed by determination of
augmentation index (AI). Measurements were performed in the fasting
state, 1, 2 and 3 h after the M or the W load. Total plasma homocysteine
(HCY) levels did not change after W administration, while M
administration resulted in a significant increase in HCY concentrations
at 3 h. FMD throughout the experiment expressed as area under the curve
(AUC) was significantly lower after the M than after the W load.
Consistent with impairment in endothelial function, the AUC of AI was
significantly higher after the M than after the W administration. Acute
HHCY impairs endothelial function and increases arterial stiffness in
patients with T2DM. This effect is probably mediated by a reduction of
nitric oxide bioavailability in endothelium.
ER -