TY - JOUR
TI - Multicenter Study for Defining the Breakpoint for Rifampin Resistance in
Neisseria meningitidis by rpoB Sequencing
AU - Taha, Muhamed-Kheir
AU - Hedberg, Sara Thulin
AU - Szatanik, Marek and
AU - Hong, Eva
AU - Ruckly, Corinne
AU - Abad, Raquel
AU - Bertrand, Sophie and
AU - Carion, Francoise
AU - Claus, Heike
AU - Corso, Alejandra
AU - Enriquez,
AU - Rocio
AU - Heuberger, Sigrid
AU - Hryniewicz, Waleria
AU - Jolley, Keith A.
AU - and Kriz, Paula
AU - Mollerach, Marta
AU - Musilek, Martin
AU - Neri,
AU - Arianna
AU - Olcen, Per
AU - Pana, Marina
AU - Skoczynska, Anna and
AU - Sorhouet Pereira, Cecilia
AU - Stefanelli, Paola
AU - Tzanakaki, Georgina
AU - and Unemo, Magnus
AU - Vazquez, Julio A.
AU - Vogel, Ulrich
AU - Wasko,
AU - Izabela
JO - Antimicrobial Agents and Chemotherapy
PY - 2010
VL - 54
TODO - 9
SP - 3651-3658
PB - AMER SOC MICROBIOLOGY
SN - 0066-4804, 1098-6596
TODO - 10.1128/AAC.00315-10
TODO - null
TODO - Identification of clinical isolates of Neisseria meningitidis that are
resistant to rifampin is important to avoid prophylaxis failure in
contacts of patients, but it is hindered by the absence of a breakpoint
for resistance, despite many efforts toward standardization. We examined
a large number (n = 392) of clinical meningococcal isolates, spanning 25
years (1984 to 2009), that were collected in 11 European countries,
Argentina, and the Central African Republic. The collection comprises
all clinical isolates with MICs of >= 0.25 mg/liter (n = 161) received
by the national reference laboratories for meningococci in the
participating countries. Representative isolates displaying rifampin
MICs of <0.25 mg/liter were also examined (n = 231). Typing of isolates
was performed, and a 660-bp DNA fragment of the rpoB gene was sequenced.
Sequences differing by at least one nucleotide were defined as unique
rpoB alleles. The geometric mean of the MICs was calculated for isolates
displaying the same allele. The clinical isolates displaying rifampin
MICs of >1 mg/liter possessed rpoB alleles with nonsynonymous mutations
at four critical amino acid residues, D542, H552, S548, and S557, that
were absent in the alleles found in all isolates with MICs of <= 1
mg/liter. Rifampin-susceptible isolates could be defined as those with
MICs of <= 1 mg/liter. The rpoB allele sequence and isolate data have
been incorporated into the PubMLST Neisseria database
(http://pubmlst.org/neisseria/). The rifampin-resistant isolates
belonged to diverse genetic lineages and were associated with lower
levels of bacteremia and inflammatory cytokines in mice. This biological
cost may explain the lack of clonal expansion of these isolates.
ER -