TY - JOUR
TI - Novel measurements of mammary stem cells in human umbilical cord blood
as prospective predictors of breast cancer susceptibility in later life
AU - Qiu, L.
AU - Low, H. P.
AU - Chang, C. -I.
AU - Strohsnitter, W. C. and
AU - Anderson, M.
AU - Edmiston, K.
AU - Adami, H. -O.
AU - Ekbom, A.
AU - Hall,
AU - P.
AU - Lagiou, P.
AU - Trichopoulos, D.
AU - Hsieh, C. -C.
JO - Annals of Oncology
PY - 2012
VL - 23
TODO - 1
SP - 245-250
PB - Oxford University Press
SN - 0923-7534, 1569-8041
TODO - 10.1093/annonc/mdr153
TODO - epithelial cell adhesion molecule; flow cytometry; hematopoietic stem
cell; integrins; in utero environment; prenatal origin
TODO - Background: The size of the breast stem-cell pool could underlie the
intrauterine roots of breast cancer. We studied whether breast stem
cells exist in umbilical cord blood and if they correlate with
hematopoietic stem-cell measurements that have been positively
associated with perinatal risk factors for breast cancer.
Subjects and methods: We isolated mononuclear cells from umbilical cord
blood of 170 singleton full-term pregnancies and determined, by reverse
transcription polymerase chain reaction, the presence of genes of
putative breast epithelial stem-cell/progenitor markers [including
epithelial cell adhesion molecule (EpCAM), CD49f (alpha 6-integrin),
CD117 (c-kit receptor), CD24, and CD29 (beta 1-integrin)]. By
immunocytochemistry, we colocalized protein expressions of
EpCAM(+)CD49f(+), CD49f(+)CD24(+), and CD24(+)CD29(+). We correlated
concentrations of putative breast stem-cell/progenitor subpopulations,
quantified by flow cytometry, with concentrations of hematopoietic stem
cells.
Results: Mammary stem-cell phenotypes were identified in umbilical cord
blood. The measured EpCAM(+) subpopulation was positively correlated
with concentrations of CD34(+) and CD34(+) CD38(-) hematopoietic stem
cells (both P = 0.006). Additionally, EpCAM(+)CD49f(+) and
CD49f(+)CD24(+) subpopulations were positively correlated to the CD34(+)
cells (P = 0.03 and 0.008, respectively).
Conclusion: The positive association between measurable breast and
hematopoietic stem cells in human umbilical cord blood suggests
plausible mechanisms for a prenatal influence on breast cancer risk.
ER -