TY - JOUR
TI - The Effect of Synbiotics on Acute Radiation-Induced Diarrhea and Its
Association with Mucosal Inflammatory and Adaptive Responses in Rats
AU - Spyropoulos, Basileios G.
AU - Theodoropoulos, George
AU - Misiakos,
AU - Evangelos P.
AU - Stoidis, Christos N.
AU - Zapatis, Haralambos and
AU - Diamantopoulou, Kalliopi
AU - Gialeli, Chrisostomi
AU - Karamanos, Nikos
AU - K.
AU - Karatzas, Gabriel
AU - Machairas, Anastasios
AU - Fotiadis,
AU - Constantinos
AU - Zografos, George C.
AU - Kelekis, Nikolaos and
AU - Kouloulias, Vasileios
JO - Digestive Diseases and Sciences
PY - 2013
VL - 58
TODO - 9
SP - 2487-2498
PB - Springer-Verlag
SN - 0163-2116, 1573-2568
TODO - 10.1007/s10620-013-2700-3
TODO - Acute radiation-induced diarrhea; Synbiotics; Radiation mucositis;
Mucosal adaptation
TODO - Background Previous clinical studies advocated that probiotics
beneficially affect acute radiation-induced diarrhea. These encouraging
results were attributed to the restoration of the intestinal flora;
however, there is lack of evidence if and how probiotics influence the
underlying pathophysiological mechanisms.
Aims The present study was conducted to investigate the potential
supporting role of a synbiotic preparation (combination of pro- and
pre-biotics) on experimentally-induced acute radiation diarrhea from the
perspective of mucosal inflammation and histological injury.
Methods Ninety adult Wistar rats were randomly assigned into six groups.
Group A (non-irradiated), group B (non-irradiated/synbiotic
supplemented), group C (irradiated), and group D (irradiated/synbiotic
supplemented) were followed up to a week after the beginning of the
experiment. Group E (irradiated) and group F (irradiated/synbiotic
supplemented) were followed up for four days. On the last day of the
experiments tissues were harvested for structural and molecular
assessments.
Results Synbiotic administration could not avert the occurrence of
diarrhea, but significantly attenuated its severity. This effect was
associated with the significant downregulation of neutrophil
accumulation and lipid peroxidation during the acute phase. During the
subacute phase, synbiotic treatment significantly improved both the
histological profile and radiation mucositis. These mechanisms
significantly contributed to the rehabilitation of the intestinal
absorptive function as further indicated from the significantly reduced
weight loss.
Conclusions Given the optimization of the intestinal flora exerted by
synbiotics, the resolution of diarrhea relies on the suppression of the
“reactive” and the augmentation of “regenerative” components of
acute radiation-induced intestinal response.
ER -