TY - JOUR
TI - Dissemination of bla(VIM) in Greece at the peak of the epidemic of
2005-2006: clonal expansion of Klebsiella pneumoniae clonal complex 147
AU - Hasan, C. M.
AU - Turlej-Rogacka, A.
AU - Vatopoulos, A. C.
AU - Giakkoupi,
AU - P.
AU - Maatallah, M.
AU - Giske, C. G.
JO - Clinical Microbiology and Infection
PY - 2014
VL - 20
TODO - 1
SP - 34-37
PB - Wiley-Blackwell
SN - 1198-743X
TODO - 10.1111/1469-0691.12187
TODO - MLST; clonal complex; metallo–lactamase; AmpC; plasmid replicon
TODO - VIM-producing Klebsiella pneumoniae (n=21) isolated from ten Greek
hospitals during 2003-2007 were characterized with multilocus sequence
typing (MLST), semi-automated repetitive sequence-based PCR (rep-PCR)
(Diversilab), plasmid replicon typing, serotyping and screening for
multiple resistance determinants. The isolates were selected to
represent different strain clusters (defined by >80% similarity)
according to pulsed-field gel electrophoresis. MLST identified three
major clonal complexes (CCs); CC147 (n=8), CC18 (n=5) and CC14 (n=3).
Plasmid replicon typing showed that IncA/C and/or IncFII(K) replicons
were detected among isolates in each of the major CCs. Good concordance
was observed between semi-automated-rep PCR genotyping and MLST.
ER -