TY - JOUR
TI - Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic
leukemia in a Spanish multicentric case-control study
AU - Casabonne, Delphine
AU - Benavente, Yolanda
AU - Robles, Claudia and
AU - Costas, Laura
AU - Alonso, Esther
AU - Gonzalez-Barca, Eva
AU - Tardon,
AU - Adonina
AU - Dierssen-Sotos, Trinidad
AU - Gimeno Vazquez, Eva and
AU - Aymerich, Marta
AU - Campo, Elias
AU - Castano-Vinyals, Gemma and
AU - Aragones, Nuria
AU - Pollan, Marina
AU - Kogevinas, Manolis
AU - Juwana,
AU - Hedy
AU - Middeldorp, Jaap
AU - de Sanjose, Silvia
JO - Infectious Agents and Cancer
PY - 2015
VL - 10
TODO - null
SP - null
PB - BMC
SN - 1750-9378
TODO - 10.1186/1750-9378-10-5
TODO - Chronic lymphocytic leukemia; Epstein-Barr virus; Serology;
Case-control; Smoking
TODO - Background: Epstein-Barr virus (EBV)-related malignancies harbour
distinct serological responses to EBV antigens. We hypothesized that EBV
serological patterns can be useful to identify different stages of
chronic lymphocytic leukemia.
Methods: Information on 150 cases with chronic lymphocytic leukemia and
157 frequency-matched (by age, sex and region) population-based controls
from a Spanish multicentre case-control study was obtained. EBV
immunoglobulin G serostatus was evaluated through a peptide-based ELISA
and further by immunoblot analysis to EBV early antigens (EA), nuclear
antigen (EBNA1), VCA-p18, VCA-p40 and Zebra. Two independent individuals
categorized the serological patterns of the western blot analysis.
Patients with very high response and diversity in EBV-specific
polypeptides, in particular with clear responses to EA-associated
proteins, were categorized as having an abnormal reactive pattern
(ab_EBV). Adjusted odds ratios (OR) and 95% confidence interval (CI)
were estimated using logistic regression models.
Results: Almost all subjects were EBV-IgG positive (>95% of cases and
controls) whereas ab_EBV patterns were detected in 23% of cases (N =
34) and 11% of controls (N = 17; OR: 2.44, 95% CI, 1.29 to 4.62; P =
0.006), particularly in intermediate/high risk patients. Although based
on small numbers, the association was modified by smoking with a gradual
reduction of ab_EBV-related OR for all Rai stages from never smokers to
current smokers.
Conclusions: Highly distinct EBV antibody diversity patterns revealed by
immunoblot analysis were detected in cases compared to controls,
detectable at very early stages of the disease and particularly among
non smokers. This study provides further evidence of an abnormal
immunological response against EBV in patients with chronic lymphocytic
leukemia.
ER -