TY - JOUR
TI - The concordance of the limiting antigen and the Bio-Rad avidity assays
in persons from Estonia infected mainly with HIV-1 CRF06_cpx
AU - Huik, Kristi
AU - Soodla, Pilleriin
AU - Pauskar, Merit
AU - Owen, S.
AU - Michele
AU - Luo, Wei
AU - Murphy, Gary
AU - Jogeda, Ene-Ly
AU - Kallas,
AU - Eveli
AU - Rajasaar, Heli
AU - Avi, Radko
AU - Masciotra, Silvina and
AU - Lutsar, Irja
AU - Del Amo, Julia
AU - Meyer, Laurence
AU - Bucher, Heiner
AU - C.
AU - Chene, Genevieve
AU - Hamouda, Osamah
AU - Pillay, Deenan and
AU - Prins, Maria
AU - Rosinska, Magda
AU - Sabin, Caroline
AU - Touloumi, Giota
AU - and Porter, Kholoud
AU - Olson, Ashley
AU - Cartier, Andrea
AU - Fradette,
AU - Lorraine
AU - Walker, Sarah
AU - Babiker, Abdel
AU - De Luca, Andrea and
AU - Fisher, Martin
AU - Muga, Roberto
AU - Kelleher, Tony
AU - Cooper, David
AU - and Grey, Pat
AU - Finlayson, Robert
AU - Bloch, Mark
AU - Ramacciotti, Tim
AU - and Gelgor, Linda
AU - Smith, Don
AU - Zangerle, Robert
AU - Gill, John and
AU - Dabis, Francois
AU - Thiebaut, Rodolphe
AU - Costagliola, Dominique and
AU - Guiguet, Marguerite
AU - Vanhems, Philippe
AU - Chaix, Marie-Laure and
AU - Ghosn, Jade
AU - Boufassa, Faroudy
AU - Meixenberger, Karolin
AU - Bannert,
AU - Norbert
AU - Bartmeyer, Barbara
AU - Antoniadou, Anastasia
AU - Chrysos,
AU - Georgios
AU - Daikos, Georgios L.
AU - Pantazis, Nikos
AU - Katsarou, Olga
AU - and Rezza, Giovanni
AU - Dorrucci, Maria
AU - Monforte, Antonella
AU - d'Arminio
AU - Geskus, Ronald
AU - van der Helm, Jannie
AU - Schuitemaker,
AU - Hanneke
AU - Sannes, Mette
AU - Dyrhol-Riise, Anne Ma
AU - Kran, Anne-Marte
AU - Bakken
AU - Rosinska, Magdalena
AU - Tor, Jordi
AU - de Olalla, Patricia
AU - Garcia
AU - Cayla, Joan
AU - del Amo, Julia
AU - Moreno, Santiago and
AU - Monge, Susana
AU - del Romero, Jorge
AU - Perez-Hoyos, Santiago and
AU - Sonnerborg, Anders
AU - Guenthard, Huldrych
AU - Scherrer, Alexandra and
AU - Malyuta, Ruslan
AU - Johnson, Anne
AU - Phillips, Andrew
AU - Morrison,
AU - Charles
AU - Salata, Robert
AU - Mugerwa, Roy
AU - Chipato, Tsungai and
AU - Price, Matt A.
AU - Gilmour, Jill
AU - Kamali, Anatoli
AU - Karita, Etienne
AU - and Burns, Fiona
AU - Giaquinto, Carlo
AU - Grarup, Jesper
AU - Kirk, Ole
AU - and Bailey, Heather
AU - Anne, Alain Volny
AU - Panteleev, Alex and
AU - Thorne, Claire
AU - Aboulker, Jean-Pierre
AU - Albert, Jan
AU - Asandi,
AU - Silvia
AU - De Wit, Stephane
AU - Reiss, Peter
AU - Gatell, Jose and
AU - Karpov, Igor
AU - Ledergerber, Bruno
AU - Lundgren, Jens
AU - Moller, Claus
AU - and Rakhma-nova, Aza
AU - Rockstroh, Juergen
AU - Sandhu, Manjinder and
AU - Dedes, Nikos
AU - Fenton, Kevin
AU - Pizzuti, David
AU - Vitoria, Marco and
AU - Faggion, Silvia
AU - Frost, Richard
AU - Raben, Dorthe
AU - Schwimmer,
AU - Christine
AU - Scott, Martin
AU - CASCADE Collaboration EuroCoord
JO - PLOS ONE
PY - 2019
VL - 14
TODO - 5
SP - null
PB - Public Library of Science
SN - null
TODO - 10.1371/journal.pone.0217048
TODO - null
TODO - Background
Serological assays to determine HIV incidence have contributed to
estimates of HIV incidence, monitoring of HIV spread, and evaluation of
prevention strategies. Two frequently used incidence assays are the
Sedia HIV-1 LAg-Avidity EIA (LAg) and the Bio-Rad avidity incidence
(BRAI) assays with a mean duration of recent infection (MDRI) of 130 and
240 days for subtype B infections, respectively. Little is known about
how these assays perform with recombinant HIV-1 strains. We evaluated
the concordance of these assays in a population infected mainly with
HIV-1 CRF06_cpx.
Material/Methods
Remnant serum samples (n = 288) collected from confirmed,
newly-diagnosed HIV-positive persons from Estonia in 2013 were tested.
Demographic and clinical data were extracted from clinical databases.
LAg was performed according to the manufacturer’s protocol and BRAI
testing was done using a validated protocol. Samples with LAg-pending or
BRAI-invalid results were reclassified as recent if they were from
persons with viral loads <1000 copies/mL or were reclassified as
long-term if presenting with AIDS.
Results
In total 325 new HIV infections were diagnosed in 2013 in Estonia. Of
those 276 persons were tested with both LAg and BRAI. Using assay
results only, the recency rate was 44% and 70% by LAg and BRAI,
respectively. The majority of samples (92%) recent by LAg were recent
by BRAI. Similarly, 89% of samples long-term by BRAI were long-term by
LAg. After clinical information was included in the analysis, the
recency rate was 44% and 62% for LAg and BRAI, respectively. The
majority of samples (86%) recent by LAg were recent by BRAI and 91% of
long-term infections by BRAI were long-term by LAg.
Conclusions
Comparison of LAg and BRAI results in this mostly CRF06_cpx-infected
population showed good concordance for incidence classification. Our
finding of a higher recency rate with BRAI in this population is likely
related to the longer MDRI for this assay.
ER -