TY - JOUR
TI - Database establishment for the secondary fungal DNA barcode
translational elongation factor 1 alpha (TEF1 alpha)
AU - Meyer, Wieland
AU - Irinyi, Laszlo
AU - Minh Thuy Vi Hoang
AU - Robert,
AU - Vincent
AU - Garcia-Hermoso, Dea
AU - Desnos-Ollivier, Marie
AU - Yurayart,
AU - Chompoonek
AU - Tsang, Chi-Ching
AU - Lee, Chun-Yi
AU - Woo, Patrick C. Y.
AU - and Pchelin, Ivan Mikhailovich
AU - Uhrlass, Silke
AU - Nenoff, Pietro and
AU - Chindamporn, Ariya
AU - Chen, Sharon
AU - Hebert, Paul D. N.
AU - Sorrell,
AU - Tania C.
AU - Halliday, Catriona
AU - Arthur, Ian
AU - Moretti, Maria Luiza
AU - and de Almeida Soares, Celia Maria
AU - Muniz, Mauro de Medeiros and
AU - Zancope-Oliveira, Rosely Maria
AU - Cruz, Fundacao Oswaldo
AU - Melo,
AU - Analy Salles de Azevedo
AU - Colombo, Arnaldo L.
AU - Nishikaku, Angela
AU - Satie
AU - Hendrickx, Marijke
AU - Stubbe, Dirk
AU - Normand, Anne-Cecile
AU - and Piarroux, Renaud
AU - Ranque, Stephane
AU - Dromer, Francoise and
AU - Arabatzis, Michael
AU - Velegraki, Aristea
AU - Cardinali, Gianluigi and
AU - Castanon, Laura Rosio
AU - Taylor, Maria Lucia
AU - Toriello, Conchita and
AU - de Hoog, Sybren
AU - Pais, Celia
AU - de Beer, Wilhelm
AU - Gryzenhout,
AU - Marieka
AU - Guarro, Josep
AU - Cano-Lira, Jose F.
AU - Robbertse, Barbara
AU - and Schoch, Conrad
AU - ISHAM Barcoding Pathogenic Fungi
JO - LIFESTYLE GENOMICS
PY - 2019
VL - 62
TODO - 3, SI
SP - 160-169
PB - Canadian Science Publishing
SN - null
TODO - 10.1139/gen-2018-0083
TODO - fungal DNA barcoding; secondary fungal DNA barcode database;
translational elongation factor 1 alpha
TODO - With new or emerging fungal infections, human and animal fungal
pathogens are a growing threat worldwide. Current diagnostic tools are
slow, non-specific at the species and subspecies levels, and require
specific morphological expertise to accurately identify pathogens from
pure cultures. DNA barcodes are easily amplified, universal, short
species-specific DNA sequences, which enable rapid identification by
comparison with a well-curated reference sequence collection. The
primary fungal DNA barcode, ITS region, was introduced in 2012 and is
now routinely used in diagnostic laboratories. However, the ITS region
only accurately identifies around 75% of all medically relevant fungal
species, which has prompted the development of a secondary barcode to
increase the resolution power and suitability of DNA barcoding for
fungal disease diagnostics. The translational elongation factor 1 alpha
(TEF1 alpha) was selected in 2015 as a secondary fungal DNA barcode, but
it has not been implemented into practice, due to the absence of a
reference database. Here, we have established a quality-controlled
reference database for the secondary barcode that together with the
ISHAM-ITS database, forms the ISHAM barcode database, available online
at http://its.mycologylab.org/. We encourage the mycology community for
active contributions.
ER -