TY - JOUR
TI - Voluntary physical activity mitigates alveolar bone loss in mice with ligature-induced experimental periodontitis
AU - Bertolini, N.O.
AU - Pereira, G.J.S.
AU - Silva, V.O.
AU - de Molon, R.S.
AU - Morari, J.
AU - Velloso, L.A.
AU - Andrade, E.F.
AU - Pereira, L.J.
AU - Moura, R.F.
JO - ARCHIVES OF ORAL BIOLOGY
PY - 2022
VL - 140
TODO - null
SP - null
PB - Elsevier Ireland Ltd
SN - 0003-9969
TODO - 10.1016/j.archoralbio.2022.105451
TODO - null
TODO - Objective: To investigate the effect of voluntary physical activity (VPA) on inflammatory profile and the progression of experimental periodontal disease (PD) in mice. Methods: Male C57BL/6 mice were randomly distributed into Control; VPA; PD and PD/VPA groups. We registered VPA (total volume of revolutions) and average speed (revolutions/minute) in a free running wheel for 30 days. On the 15th day, animals from the PD and PD/VPA groups received ligatures on the upper second molars bilaterally. On the 30th day animals were euthanized, and PD progression was assessed by measuring alveolar bone loss (ABL - the linear distance between the cemento-enamel junction and the alveolar bone crest on the teeth buccal surface). Gene expression of RANKL (kappa nuclear factor B receptor) OPG (osteoprotegerin), IL-1β (interleukin 1 beta), IL-6 (interleukin 6) and TNF-α (tumor necrosis factor alpha) were evaluated by real-time PCR (quantitative Polymerase Chain Reaction - relative gene expression). Results: The total volume of physical activity and the activity speed decreased along the seven days after ligature-placement (p < 0.05), returning to a similar pattern in relation to VPA group. Ligature placement produced significant bone resorption, and increased RANKL, IL-1β, IL-6 and TNF-α expression. VPA reduced ABL (p < 0,05) and the expression of TNF-α and IL-1β, whereas increased OPG expression. Conclusion: Animals induced to PD with access to the VPA wheel presented both lower gingival inflammation and less alveolar bone resorption in comparison to animals without access to the wheel. © 2022 Elsevier Ltd
ER -