TY - JOUR
TI - Photosynthetic epibionts and endobionts of Pacific oyster shells from oyster reefs in rocky versus mudflat shores
AU - Barillé, L.
AU - Le Bris, A.
AU - Méléder, V.
AU - Launeau, P.
AU - Robin, M.
AU - Louvrou, I.
AU - Ribeiro, L.
JO - PLOS ONE
PY - 2017
VL - 12
TODO - 9
SP - null
PB - Public Library of Science
SN - null
TODO - 10.1371/journal.pone.0185187
TODO - algal growth;  animal shell;  Article;  bacterial colonization;  bacterial phenomena and functions;  bacterium assemblage;  biochemical analysis;  biomass;  biophysics;  controlled study;  Crassostrea gigas;  cyanobacterium;  diatom;  epipelic;  Eugomontia testarum;  green alga;  high performance liquid chromatography;  Hippodonta;  Hyella caespistosa;  image analysis;  Leptolyngbya terrebrans;  lipophilicity;  Mastigocoleus testarum;  motile epipsammic;  mudflat shore;  Navicula;  Nitzschia;  nonhuman;  Normalized Difference Vegetation Index;  Ostreobium quekettii;  osyter reef;  photosynthetic endobiont;  photosynthetic epibiont;  phototrophic bacterium;  rating scale;  red alga;  rocky shore;  seashore;  spatial analysis;  species diversity;  spectroscopy;  taxonomy;  tychoplankton;  vegetation;  animal;  biodiversity;  classification;  Crassostrea;  metabolism;  photosynthesis;  physiology;  pigmentation;  plant;  symbiosis, Animals;  Biodiversity;  Biomass;  Crassostrea;  Photosynthesis;  Pigmentation;  Plants;  Symbiosis
TODO - The Pacific oyster, Crassostrea gigas (Thunberg), is the main bivalve species cultivated in the world. With global warming enabling its reproduction and larval survival at higher latitudes, this species is now recognized as invasive and creates wild oyster reefs globally. In this study, the spatial distribution of photosynthetic assemblages colonizing the shells of wild C. gigas was investigated on both a large scale (two contrasting types of reefs found in mudflats and rocky areas) and a small scale (within individual shells) using a hyperspectral imager. The microspatial distribution of all phototrophs was obtained by mapping the Normalized Difference Vegetation Index (NDVI). Second derivative (δδ) analyses of hyperspectral images at 462, 524, 571 and 647 nm were subsequently applied to map diatoms, cyanobacteria, rhodophytes and chlorophytes, respectively. A concomitant pigment analysis was carried out by high performance liquid chromatography and completed by taxonomic observations. This study showed that there was high microalgal diversity associated with wild oyster shells and that there were differences in the structure of the phototropic assemblages depending on the type of reef. Namely, vertically-growing oysters in mudflat areas had a higher biomass of epizoic diatoms (hyperspectral proxy at δδ462 nm) and were mainly colonized by species of the genera Navicula, Nitzschia and Hippodonta, which are epipelic or motile epipsammic. The assemblages on the horizontal oysters contained more tychoplanktonic diatoms (e.g. Thalassiosira pseudonana, T. proschkinae and Plagiogrammopsis vanheurckii). Three species of boring cyanobacteria were observed for both types of reef: Mastigocoleus testarum, Leptolyngbya terrebrans, and Hyella caespistosa, but the second derivative analysis at 524 nm showed a significantly higher biomass for the horizontally-growing oysters. There was no biomass difference for the boring chlorophyte assemblages (δδ647 nm), with two species: Eugomontia testarum and Ostreobium quekettii observed for both types of reef. This study shows that oyster shells are an idiosyncratic but ubiquitous habitat for phototrophic assemblages. The contribution of these assemblages in terms of biomass and production to the functioning of coastal areas, and particularly to shellfish ecosystems, remains to be evaluated. © 2017 Barillé et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ER -