@article{3088141,
    title = "Expression analysis of mir-17-5p, mir-20a and let-7a microRNAs and their target proteins in CD34+ bone marrow cells of patients with myelodysplastic syndromes",
    author = "Vasilatou, D. and Papageorgiou, S.G. and Kontsioti, F. and Kontos, C.K. and Tsiotra, P. and Mpakou, V. and Pavlou, M.A.S. and Economopoulou, C. and Dimitriadis, G. and Dervenoulas, J. and Pappa, V.",
    journal = "Leukemia Research",
    year = "2013",
    volume = "37",
    number = "3",
    pages = "251-258",
    issn = "0145-2126",
    doi = "10.1016/j.leukres.2012.11.011",
    keywords = "cyclin D1;  K ras protein;  microRNA;  microRNA 17 5p;  microRNA 20a;  microRNA let 7a;  Myc protein;  protein bcl 2;  transcription factor E2F1;  unclassified drug, adult;  article;  bone marrow cell;  CD34 selection;  clinical article;  controlled study;  cytogenetics;  down regulation;  female;  high risk patient;  human;  human cell;  karyotype;  low risk population;  male;  myelodysplastic syndrome;  overall survival;  priority journal;  prognosis;  protein expression;  protein targeting;  real time polymerase chain reaction;  Western blotting, Aged;  Aged, 80 and over;  Antigens, CD34;  Bone Marrow Cells;  Case-Control Studies;  Female;  Gene Expression Profiling;  Gene Expression Regulation;  Humans;  Male;  MicroRNAs;  Middle Aged;  Myelodysplastic Syndromes;  Prognosis",
    abstract = "Mir-17-5p and mir-20a, members of the mir-17-92 family, down-regulate E2F1, which is over-expressed in myelodysplastic syndromes (MDS). Moreover, let-7a down-regulates KRAS, which is aberrantly expressed in MDS. We evaluated the expression of the aforementioned microRNAs in CD34+ cells of 43 MDS patients using real-time PCR and their target proteins (E2F1, MYC, BCL2, CCND1, and KRAS) by Western blot. Mir-17-5p and mir-20a were under expressed in high risk MDS patients, compared to low risk MDS patients. Similarly, let-7a was under expressed in patients with intermediate or high-risk karyotype. Interestingly, there was an inverse correlation between microRNA and the expression levels of their targets. Importantly, mir-17-5p and mir-20a constitute favorable prognostic factors in MDS, since their expression was associated with increased overall survival of MDS patients. © 2012 Elsevier Ltd."
}