Biological activities of plant derived substances in nuclear receptors

Doctoral Dissertation uoadl:1305861 483 Read counter

Τομέας Βασικών Επιστημών
Library of the School of Health Sciences
Deposit date:
Κουνάδη Σταματίνα
Dissertation committee:
Αναπλ. Καθηγήτρια Χριστίνα Κανακά Gantenbein
Original Title:
Βιολογικές δράσεις ουσιών φυτικής προέλευσης σε πυρηνικούς υποδοχείς
Translated title:
Biological activities of plant derived substances in nuclear receptors
At the present thesis phytochemical analysis of endemic plants Ebenus cretica,
Ebenus sibthorpi, Medicago marina and Medicago falcata using cutting-edge
technologies (ASE, FCPC, NMR) was conducted. Isolated substances were tested in
cell lines for estrogenic /anti-estrogenic and anti-inflammatory activity. From
E.cretica eleven substances were isolated belonging to the class of flavonoids
whereas from M.marina four saponins. Ombuoside, maesopsin and two bayogenin
glycosides (MMRTK23 & 25) were selected for further control.
In order to investigate the mechanism of ombuoside, maesopsin and MMRTK23 in
vitro assays generally accepted for their ability to reflect the estrogenicity
of a substance were used. The effect of the compounds on the IGFBP-3 protein
and the PS2 gene was tested in breast cancer cells (MCF-7). Osteoblasts KS483
were tested for the increased creation of mineralized nodules. Furthermore in
vivo study of the total methanolic extract of E.cretica in ovariectomized
Wistar female rats was performed. According to the results the ombuoside and
maesopsin exhibit mild estrogenic activity which is engaged through the
estrogen receptors.
In order to gain further insight of the activity of the saponins in the
inflammation process PC-3 prostate cancer cells were used. The possible effect
of saponins on the cell viability of PC-3 and on the translation of the
interleukin IL-6 was investigated. The results showed that at all
concentrations and dose-dependently the MMRTK23 and 25 reduce the expression of
interleukin, however, their action is not performed through the transcription
factor NFkB.
Flavonoids, Estrogenicity, Saponins, Breast cancer, Prostate cancer
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