Genetic markers (HLA antigens, adhesion molecules and co-stimulatory molecules) in colorectal cancer. Cytometric, serological and histological study

Doctoral Dissertation uoadl:1305898 168 Read counter

Unit:
Τομέας Χειρουργικής
Library of the School of Health Sciences
Deposit date:
2013-09-04
Year:
2013
Author:
Καρβούνης Ευάγγελος
Dissertation committee:
Καθηγητής Βώρος Διονύσης, Καθηγητής Οικονομόπουλος Θεοφάνης, Επίκουρος Καθηγητής Γκρινιάτσος Ιωάννης
Original Title:
Γενετικοί δείκτες (HLA αντιγόνα, μόρια προσκόλλησης και συνδιέγερσης) σε καρκίνο παχέος εντέρου. Κυτταρική, ορολογική, ιστολογική μελέτη
Languages:
Greek
Translated title:
Genetic markers (HLA antigens, adhesion molecules and co-stimulatory molecules) in colorectal cancer. Cytometric, serological and histological study
Summary:
Aim: To investigate the expression of genetic markers that are likely to be
involved in the pathogenesis and expansion of colon cancer.
Materials-Methods: 133 patients with colorectal carcinoma were included. The
study involved I) HLA class I, II antigens typing in patients and healthy
donors, II) analysis of soluble adhesion molecules, III) evaluation of
expression of HLA and other molecules in cancer, dysplasia and normal
epithelium histologically, IV) flow cytometry for cell sub-populations, in the
tumor and in the healthy boundaries
Results: α) increased expression of HLA-Cw3, HLA-Cw7, HLA-DR4, HLA-DR16 in
comparison to healthy volunteers. Increased HLA-Cw3 in patients in stages C, D
β) increased expression of soluble E-cadherin in the serum of cancer patients
was co-related with smaller size of tumor. Elevated levels of s-VCAM and s-ICAM
were seen in the reactive fibrosis surrounding area c) histologically, reduced
E-cadherin, CD80, CD86 and increased HLA-DR in cancer cells in comparison to
the surrounding healthy boundaries d) flow cytometric analysis revealed
increased HLA-ABC in cancer cells from patients with increased tumor size in
comparison to healthy tissue.
Conclusion: The findings of this study reinforce the idea that the immune
system through a variety of factors, including the markers tested, can affect
growth and metastatic spread of colon cancer.
Keywords:
Colorectal cancer, Genetic markers, HLA antigens, Adhesion molecules, Co-stimulatory molecules
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
200
Number of pages:
195

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