Εκτίμηση της λειτουργικής ικανότητας των ειδικών για τον HIV CD4 και CD8 λεμφοκυττάρων στη διάρκεια της λοίμωξης προ και κατά τη διάρκεια της θεραπείας (HAART).

Doctoral Dissertation uoadl:1306122 499 Read counter

Unit:
Τομέας Κλινικοεργαστηριακός
Library of the School of Health Sciences
Deposit date:
2013-03-28
Year:
2013
Author:
Χατζοπούλου Δήμητρα
Dissertation committee:
Καθηγητής Ράπτης Σωτήρης, Καθηγητής Δερβενούλας Ιωάννης, Αναπλ. Καθηγήτρια Μπουτάτη Ελένη
Original Title:
Εκτίμηση της λειτουργικής ικανότητας των ειδικών για τον HIV CD4 και CD8 λεμφοκυττάρων στη διάρκεια της λοίμωξης προ και κατά τη διάρκεια της θεραπείας (HAART).
Languages:
Greek
Summary:
The purpose of this study is the evaluation of the specific immune response in
HIV+ patients. The functional capacity of HIV specific CD4+ and CD8+ T
lymphocytes in HIV infection and their role in therapy was assessed via the use
of tetramers and intracellular cytokines detection by flow cytometric analysis.
Samples from 94 HIV+ patients were taken. The detection of HIV-specific CD8+ T
lymphocytes via the use of tetramers as well as the isolation of HIV- specific
CD4+ and CD8+ T cells via intracellular cytokine staining and flow ctometry
analysis, offer significant information concerning assessment of the specific
imuunologic response against HIV. The increased amount of HIV-specific CD27+
CD8+ T lymphocytes may suggest that they do not mature sufficiently to be able
to inhibit viral proliferation, as they fail to differentiate into fully
mature, active cells. It was also determined that anti-retroviral treatment
improves the activity of HIV-specific CD8+ T lymphocytes as regards their
ability to synthesize cytokines.
Keywords:
Tetramers, Intracellular cytokines detection, HAART, HIV infection, specific immune response
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
151
Number of pages:
V, 125
File:
File access is restricted.

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