Τομέας Χειρουργικής
Library of the School of Health Sciences

2014-06-23

2014

Βιττωράκη Αγγελική

Αλκιβιάδης Κωστάκης, Θεόδωρος Καρατζάς, Ευστάθιος Αντωνίου

Εκτίμηση της λειτουργικής κατάστασης in vitro των CD4+T κυττάρων ληπτών νεφρικού μοσχεύματος με μέτρηση της συγκέντρωσης του ενδοκυττάριου νουκλεοτιδίου adenosine triphosphate (ATP)

Greek

Evaluation in vitro of CD4+T cell function in renal transplant recipients by measuring the concentration of intracellular adenosine triphosphate (ATP)

Previous studies have shown that intracellular ATP (iATP) in activated CD4+T
cells in vitro may identify renal transplant recipients (RTRs) at risk of
infection or rejection. In this study, we evaluated whether this test could
identify the “level of risk” in 656 RTRs with good and stable graft function.
Therefore, 1095 blood samples from stable RTRs, 56 from RTRs with infection, 7
from RTRs with rejection and 200 from healthy donors-(NC) during a two-year
time were analyzed. The classification of T-cell response into strong, moderate
and low revealed significant difference between patient’s and NC’s in low and
strong responses. The great majority of patients’ samples exhibited moderate
immune response. Interestingly, 128 of the patients had fluctuated T-cell
response between the three response zones. T-cell response was increased after
time post-transplantation and was found higher in protocols using Azathioprine
versus other immunosuppression (p<0.001) and Cyclosporine instead of Tacrolimus
(p=0.012). In addition, iATP levels were associated with infection (p=0.001)
but not with rejection (p=0.068).
In conclusion, we cannot support with safety this assay as immune monitoring
test post-transplantation in clinically stable RTRs. Since T-cell activation is
mainly affected by immunosuppression this test may give information regarding
the efficacy of different immunosuppressive protocols.

Intracellular ATP, CD4+ T cells, Immunosuppression, Immune monitoring, Renal transplantation

Yes

1-2

Yes

227

iv,189

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https://pergamos.lib.uoa.gr/uoa/dl/object/1306124