Determination of urinary leukotriene levels in atopic and non-atopic preschool children after infection(virus induced asthma phenotype)

Doctoral Dissertation uoadl:1308012 190 Read counter

Unit:
Τομέας Υγείας - Μητέρας - Παιδιού
Library of the School of Health Sciences
Deposit date:
2013-04-22
Year:
2013
Author:
Μαρμαρινός Αντώνης
Dissertation committee:
Κωνσταντόπουλος Ανδρέας, Τζουμάκα-Μπακούλα Χρύσα, Σαξώνη-Παπαγεωργίου Φωτεινή, Γουργιώτης Δημήτριος, Τσολιά Μαρίζα, Γαρούφη Ευαγγελία, Πρίφτης Κωνσταντίνος
Original Title:
Προσδιορισμός λευκοτριενίων στα ούρα παιδιών με συρίττουσα αναπνοή μετά από λοίμωξη (φαινότυπο λοιμώδους άσθματος) με η χωρίς ατοπία
Languages:
Greek
Translated title:
Determination of urinary leukotriene levels in atopic and non-atopic preschool children after infection(virus induced asthma phenotype)
Summary:
Aim: To determine the levels of urinary Leukotriene E4 (U-LTE4) in atopic and
non-atopic preschool children with virus induced asthma, in an effort to assess
the relationship of urinary LTE4 to particular asthma phenotype in this age.
Method: Levels of urinary Leukotriene E4 were measured by means of Enzyme
Immunoassay (EIA) and the results were expressed in pg/mg of secreted
Creatinine in 96 preschool children (mean 3 years ) with virus induced asthma,
52 atopic and 44 non-atopic, during exacerbation and in remission. Atopy was
determined by specific serum IgE measurement and skin prick test to a panel of
common allergens. Urinary LTE4 was also measured in 36 age-matched,
non-asthmatic, non-atopic control children. The results were analyzed using the
non-parametric Mann-Whitney U test.
Results: During exacerbation, U-LTE4 was significantly higher in all children,
atopic and non-atopic, with virus-induced asthma in comparison to A.
Remission: 642.20±268 vs. 399.45±204, P value <0.001 and B. Controls:
642.20±268 vs 271.39±83 P value <0.001. Atopic patients demonstrated
significantly higher levels of U-LTE4 in relation to non atopic, both during
exacerbation (872.13±246 vs 613.15±150 P value=0.0013) and in the remission
phase (507.59±182 vs 283.59±160 P value <0.001). During remission, a highly
significant difference of U-LTE4 was found to persist when controls were
compared to atopic patients: 271.39±83 vs 507.59±182 P value=0.002 but not when
compared to non atopic ones: 271.39±83 s 283.59±160 P value=0.432. The greatest
change in the levels (drop) of U-LTE4 was within the first 50 days of the
outbreak Atopic patients never reached the baseline of the controls.
Conclusion: Urinary Leukotriene E4 (U-LTE4) is a strong indicator of virus
induced asthma exacerbation in preschool children, more so in atopics.
Increased basal levels of urinary LTE4 occur only in atopics. This suggests a
potential role of urinary LTE4 as a marker of atopic, virus induced asthma in
preschool children. Improvement in the predictive ability and formation of a
better therapeutic strategy in the treatment of asthma.
Keywords:
Leukotriene E4, Asthma, Children allergies, Asthma biomarkers, Asthma phenotypes
Index:
Yes
Number of index pages:
5,6,7
Contains images:
Yes
Number of references:
228
Number of pages:
136
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