Unit:
Τομέας Υγείας - Μητέρας - ΠαιδιούLibrary of the School of Health Sciences
Dissertation committee:
Χρούσος Γεώργιος, Μαύρου-Καλπίνη Αριάδνη, Μουτσάτσου-Λαδικού Παρασκευή
Original Title:
Πρωτεωμική ανάλυση πλάσματος υπό συνθήκες έντονου στρες
Summary:
The Spartathlon race (brisk walking a distance of 246 km in less than 36 h) was
employed as a model of severe physical stress to investigate proteomic
alterations in the plasma of athletes at the start (Athens – Phase I) and
finish (Sparta– Phase II) of the race, as well as 48h after the race (Post–
Phase III). The athletes’ plasma was analysed by 2D gel electrophoresis (2-DE)
and the differentially expressed proteins were identified by matrix-assisted
laser desorption ionisation-time of flight (MALDI-TOF) mass spectrometry
(MS). Our results were confirmed by Western blot and biochemical analyses.
Overall fifty-one proteins were differentially expressed between the starting
point, the finishing line and two days after the end of the race. Overall,
fifty-one proteins were found to be differentially expressed between the
starting line, the finishing line and two days after the completion of the
race. Fifty-one proteins were involved in the inflammatory process, while the
rest concerned anti-oxidant, anti-coagulant and metabolic processes, as well as
iron transport. Our findings suggested that prolonged physical stress affects
circulating stress-related proteins, which might be employed as biomarkers of
stress-related diseases.
Keywords:
Stress, Spartathlon, Plasma, Proteomics, Inflammation
Number of references:
204
File:
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document.pdf
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