In vitro study of the apoptotic agents- Survivin and Cytochrome c- in the pathogenesis of Juvenile Idiopathic Arthritis (JIA)

Doctoral Dissertation uoadl:1308491 81 Read counter

Unit:
Τομέας Υγείας - Μητέρας - Παιδιού
Library of the School of Health Sciences
Deposit date:
2013-04-05
Year:
2013
Author:
Βράϊλα Βενετία-Μαρία
Dissertation committee:
Καθ. Κωνσταντόπουλος Ανδρέας, Αν. Καθ. Γουργιώτης Δημήτριος, Επικ. Καθ. Κουκουτσάκης Πέτρος
Original Title:
In vitro μελέτη των αποπτωτικών παραγόντων- Survivin και Cytochrome c- στην παθογένεια της Νεανικής Ιδιοπαθούς Αρθρίτιδας (ΝΙΑ)
Languages:
Greek
Translated title:
In vitro study of the apoptotic agents- Survivin and Cytochrome c- in the pathogenesis of Juvenile Idiopathic Arthritis (JIA)
Summary:
Juvenile Idiopathic Arthritis is a chronic disease of childhood, and is
sometimes accompanied with severe clinical manifestations. Τhe exact mechanism
of pathogenesis remains unknown.
The aim of this research was the study of the mechanism of apoptosis in the
joints of patients with JIA and the investigation of its role in the
pathogenesis of the disease. For this reason two principal molecules, Survivin
which acts as an inhibitor and Cytochrome-c which induces the apoptosis, were
studied in vitro.
According to the results in this trial:
1. The mechanism of apoptosis is present in harmed joint.
2. The presence and the expression of Survivin and Cytochrome-c is
irrelevant to the clinical features of the disease. In the case of systematic
JIA the expression of Survivin and Cytochrome-c is of a lower impact.
3. The effect of these two agents, and especially of this Cytochrome-c,
expands extraarticularly, affecting Hgb levels and WBC counts.
4. Apoptosis is irrelevant to the activity and the effect of the
autoimmunity.
5. The concentration of Survivin and Cytochrome-c is affected by the
treatment with the biological agent anti-TNF, showing a reduction in its
levels.
It is obvious that the mechanism of apoptosis is active in the joint acting as
a pathogenic factor of JIA. It is showed for the first time that these two
agents, Survivin and Cytochrome-c, possibly affect the progress and the
clinical profile of the disease. The effect of the biological agents on the
concentration of these two molecules, as a response to the treatment, suggests
new promising therapeutical targets.
Keywords:
Juvenile Idiopathic Arthritis , Apoptosis , Survivin , Cytochrome C, Synovial Fluid
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
170
Number of pages:
X, 78
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