In vivo measurement of optical characteristics of precancerous lesions in the uterine cervix and correlation with quantitative pathologic parameters

Doctoral Dissertation uoadl:1308505 89 Read counter

Τομέας Υγείας - Μητέρας - Παιδιού
Library of the School of Health Sciences
Deposit date:
Χατζηπαπάς Ιωάννης
Dissertation committee:
Διακομανώλης Εμμανουήλ (επιβλέπων), Αντζακλής Αριστείδης, Μακρής Νικόλαος
Original Title:
In vivo μέτρηση δυναμικών οπτικών χαρακτηριστικών σε προκαρκινικές αλλοιώσεις του τραχηλικού επιθηλίου και συσχέτιση τους με ποσοτικοποιημένες παθολογοανατομικές παραμέτρους
Translated title:
In vivo measurement of optical characteristics of precancerous lesions in the uterine cervix and correlation with quantitative pathologic parameters
Introduction: The existing diagnostic procedures for detecting and grading
epithelial cancers and pre-cancers are qualitative, subjective, multi-step,
labour intensive and overall they are characterized by low cost effectiveness.
Dynamic Spectral Imaging (DSI) is a novel optical imaging technique that is
capable of providing improved and objective information for the tissue
pathology based on the quantitative assessment of diffuse spectral reflectance
after administration of a pathology differentiating agent. The aim of this
study was to investigate potential correlations between the optical
characteristics of the cervix, evaluated with the DSI technique, and
quantitative parameters extracted via image analysis of corresponding tissue
section images.
Methods: The cervix of 74 Caucasian women was examined using DySISTM. Dynamic
Spectral curves were collected from 148 tissue locations of the cervix after
identifying abnormal areas during colposcopy. From each curve two main
parameters were computed: maximum intensity (DSIMAX), and total area under the
curve (DSIAREA).
An equal number of tissue biopsies was also obtained from the same locations,
and computerised image analysis was performed to extract the following
histopathological parameters across each of the basal, intermediate and
superficial layers: Nuclear to Cytoplasmic Ratio (NCR), Nuclear Area (NA),
Nuclear Perimeter (NP), Longest Horizontal Segment of the nucleus (LHS), and
Elongation Factor (EF) of the nucleus. The significance of the correlation of
these parameters with the DSI parameters was investigated using the Pearson’s
Results: Among the 30 pairs of correlation, only NCR was found to be
significantly correlated with both DSI parameters, for all three layers of the
epithelium (p<0.05). No other correlation was found between any combination of
cellular and spectral parameters, for any epithelial layer.
Conclusions: Our data suggest that the DSI curves may also provide
quantitative information about the cell-packing properties of the epithelium.
Precancerous lesions , Cervical epithelial, Quantitative pathology , Dynamic spectral imaging (DSI) , Cell packing
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