Dissertation committee:
Αναπλ. Καθηγητής Νικόλαος Θωμαΐδης (επιβλέπων), Καθηγητής Μιχαήλ Κούππαρης, Αναπλ. Καθηγήτρια Ειρήνη Παντερή
Summary:
Hydrophilic Interaction Chromatography (HILIC) has found interesting
applications for the study of hydrophilic compounds.Artificial sweeteners are
widely used in food industry. The annual consumption of aspartame worldwide is
16.000 tons, while sucralose is produced in 1500 tons in America and 400 tonsin
Europe. Due to increased consumption, sweeteners are widely distributed in the
aquatic environment and are characterized as emerging
pollutants.Inthisthesis,westudythemostpopularartificialsweeteners (suchas,
aspartame, neotame, advantame, alitame, acesulfameK, saccharin, cyclamate,
sucraloseandneohesperidindihydrochalcone) with hydrophilic liquid
chromatography tandemmass spectrometry. A method for the simultaneous
determination of eight artificial sweeteners (aspartame, neotame, alitame,
acesulfame K, saccharin, cyclamate, sucralose and neohesperidin
dihydrochalcone) with HILIC-ESI-MS/MS was developed and validated in waste
water treatment plant from Athens, Greece and in drinking water from Attiki.
Furthermore, the fragmentation pathways of artificial sweeteners (aspartame,
neotame, advantame, alitame, acesulfame K, saccharin, cyclamate, sucralose and
neohesperidin dihydrochalcone)were studied by high resolution mass spectrometry
(HRMS, Orbitrap Velos) under positive and negative ESI mode. The results were
compared with those obtained using atriple quadrupole mass spectrometer.
Finally, the retention mechanism of nine artificial sweeteners was studied with
a silica column (Kinetex HILIC), soulfoalkylobetain (ZIC-HILIC), amide- (TSKgel
Amide-80) and aminopropyl- (APS-2 Hypersil) with LC-MS/MS.
Keywords:
Artificial sweeteners, HILIC, Wastewater treatment plant, LC-MS/MS, Fragmentation
Number of index pages:
10-15, 16-17, 19-26, 28-30
