Unit:
Τομέας Βιολογίας Κυττάρου Και ΒιοφυσικήςLibrary of the School of Science
Dissertation committee:
Ισιδώρα Παπασιδέρη, Καθηγήτρια Κυτταρικής και Αναπτυξιακής Βιολογίας, Τμήμα Βιολογίας Παν/μίου Αθηνών
Original Title:
Ανίχνευση, τυποποίηση και έκφραση των ογκογόνων πρωτεϊνών Ε6 και Ε7 σε δυσπλασίες και καρκίνους του κατώτερου γεννητικού και γαστρεντερικού συστήματος
Translated title:
Detection, genotyping and expression of E6 and E7 HPV oncoproteins in dysplasias and cancers of the lower genital and gastrointestinal tract
Summary:
The objective of the present study was to investigate the frequency of HPV DNA
detection, as well as mRNA of the E6/E7 oncogenes in dysplasias and cancers of
the lower genital and gastrointestinal tract. 290 tissue sections from the
lower genital and upper gastrointestinal tract were studied. DNA microarrays
were used for the HPV DNA detection and genotyping, while Real- time NASBA was
used for the detection of E6/E7 mRNA. The main finding regarding samples from
the lower genital tract was the higher detection rate of E6/E7 mRNA in
high-grade dysplasias and cancers compared to low-grade dysplasias (66.7% vs.
32.4% p<0,001 and 90.9% vs. 32.4% p<0,001, respectively for cervical samples,
0.0% vs. 60.0% p=0.05 and 0.0% vs. 100% p<0.001, respectively for vaginal
samples, 57.1% vs. 23.5% p=0.05 and 100% vs. 23.5% p= 0.001, respectively for
vulvar samples). Furthermore, it was found that 9.6% of the oral cancers and
14.3% of oropharyngeal cancers were positive for the detection of E6/E7 mRNA.
Detection of mRNA of the E6/E7 oncogenes could be a prognostic marker in
triaging lesions with increased risk of progression in the lower genital tract
and indicates a possible role of HPV in the carcinogenesis of the upper
gastrointestinal tract.
Keywords:
HPV, Oncoproteins, Lower genital tract, Upper gastrointestinal tract, mRNA detection
Number of references:
370
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